کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9757477 | 1496004 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Printing microarrays of bacteria for identification by infrared microspectroscopy
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Fourier transform infrared (FTIR) spectroscopy has been established as a rapid bacteria identification technique. To increase the throughput of this methodology, the feasibility of applying contact deposition microarray technology to print intact bacterial cells as arrayed deposits (150 μm diameter) on optical substrates and on agar slides was demonstrated for the first time. This contact deposition technology delivers nanoliter (nL) droplets of bacterial suspensions from a microtiter plate onto a slide surface. Protocols for printing microarrays of whole-cells on agar and on infrared (IR)-transparent slides were evaluated and optimized for subsequent measurement by IR microspectroscopy and IR imaging. Parameters that were investigated included pin capacity, deposition mode, and spatial distribution of microarrays. Bacteria representing eight genera (Yersinia, Staphylococcus, Salmonella, Listeria, Enterobacter, Citrobacter, Klebsiella, and Escherichia) were used in this proof-of-concept study. The resulting dendrograms generated by hierarchical cluster analysis (HCA) demonstrated clustering of the descendants of the foodborne bacteria investigated into their respective branches. The suitability of microarray printing coupled to focal-plane-array (FPA) FTIR detection for the rapid identification of bacteria was demonstrated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vibrational Spectroscopy - Volume 38, Issues 1â2, 29 July 2005, Pages 229-235
Journal: Vibrational Spectroscopy - Volume 38, Issues 1â2, 29 July 2005, Pages 229-235
نویسندگان
Magdi M. Mossoba, Sufian F. Al-Khaldi, Jonah Kirkwood, Frederick S. Fry, Jacqueline Sedman, Ashraf A. Ismail,