کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
98776 160892 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of DNAstable™ for DNA storage at ambient temperature
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Evaluation of DNAstable™ for DNA storage at ambient temperature
چکیده انگلیسی

Preserving DNA is important for validation of prospective and retrospective analyses, requiring many expensive types of equipment (e.g., freezers and back-up generators) and energy. While freezing is the most common method for storing extracted DNA evidence or well-characterized DNA samples for validation studies, DNAstable™ (Biomatrica®), a commercially available medium for room temperature storage of DNA extracts was evaluated in this study. Two groups of samples consisting of different DNA quantities were investigated, one ranging from 20 to 400 ng (group 1) and the other one ranging from 1.4 to 20 ng (group 2). The DNA samples with and without DNAstable™ were stored at four different temperatures [∼25 °C (room temperature), −20 °C, 37 °C or 50 °C]. DNA degradation over several months was monitored by SYBR® Green-based qPCR assays and by PCR amplification of the core CODIS STR markers for group 1 and 2 DNA samples, respectively. For the time points tested in this study (up to 365 days), the findings indicate that the −20 °C controls and the DNAstable™ protected samples at room temperature provided similar DNA recoveries that were higher compared to the unprotected controls kept at RT, 37 °C or 50 °C. These results suggest that DNAstable™ can protect DNA samples with effectiveness similar to that of the traditional −20 °C freezing method. In addition, extrapolations from accelerated aging experiments conducted at high temperatures support that DNAstable™ is an effective technology for preserving purified DNA at room temperature with a larger protective impact on DNA samples of low quantity (<20 ng).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 8, Issue 1, January 2014, Pages 170–178
نویسندگان
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