کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9885575 | 1537331 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Positive and negative regulatory elements in the late lactation protein-A gene promoter from the tammar wallaby (Macropus eugenii)
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کلمات کلیدی
PRLEMSACHO-K1PRLRSEAPCATBSA - BSAElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزbovine serum albumin - آلبومین سرم گاوSecreted alkaline phosphatase - آلکالن فسفاتاز تثبیت شدهChinese Hamster Ovary - تخمدان هامستر چینیTransgenic - تراریختهGene regulation - تنظیم ژنMilk protein - پروتئین شیرProlactin - پرولاکتین chloramphenicol acetyl transferase - کلرامفنیکول استیل ترانسفرازMarsupial - کیسهدارانprolactin receptor - گیرنده پرولاکتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Little is known about the regulation of the marsupial-specific late lactation protein-A (LLP-A) gene, first expressed at mid-lactation in the mammary gland of the tammar wallaby. A genomic clone of LLP-A was sequenced and shown to include seven exons. The LLP-A promoter region of 1969 bp ligated to a secreted alkaline phosphatase (SEAP) gene reporter was co-transfected into CHO-K1 cells with prolactin (PRL) receptor cDNA. Transfected cells cultured with insulin, cortisol and PRL did not secrete SEAP into media. Similarly, this construct was not expressed in the mammary gland of eight lines of transgenic mice. In contrast, when the LLP-A promoter region was reduced to 850 bp, the expression of the SEAP reporter in CHO-K1 cells was constitutive and PRL-independent, despite the presence of two low affinity Stat5 binding sites. The 1969 bp promoter was analyzed using nine serial deletions ligated to the SEAP gene. The expression of these constructs was PRL-independent. Five putative inhibitory elements were identified between â1969 and â1796, â1404 and â1184, â1184 and â992, â992 and â757, and â591 and â425, and a putative enhancer or core transcription element between â425 andâ239. These studies indicate that the complex temporal regulation of the LLP-A gene involves elements in its 5â²-regulatory region.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1728, Issues 1â2, 5 April 2005, Pages 65-76
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1728, Issues 1â2, 5 April 2005, Pages 65-76
نویسندگان
Josephine F. Trott, Timothy E. Adams, Michael Wilson, Kevin R. Nicholas,