کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9889665 | 1539171 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Participation of high-density lipoprotein in vitellogenesis in Japanese eel hepatocytes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
To investigate effect of estradiol-17β (E2) treatment in vivo on binding of eel hepatocytes to HDL, we developed hepatocytes binding assay. When hepatocytes were incubated with 200 times excess of eel HDL, the binding of hepatocytes to HDL precoated on wells was inhibited competitively. This indicates that eel hepatocytes bound specifically to HDL. E2 treatment in vivo induced vitellogenin (VTG) synthesis. Hepatocytes prepared from the same E2 treatment eel showed 3-fold higher ability of binding to HDL compared to hepatocytes prepared from ells without E2 treatment. We also examined effects of E2 and HDL on VTG induction in cultured hepatocytes. VTG, determined by enzyme-linked immunosorbent assay (ELISA), induction was about two-times higher in the presence of both 10â5 M of E2 and 400 μg of HDL than in the presence of 10â5 M E2 alone. At concentrations below 10â6 M E2, VTG was not induced in the presence or absence of HDL. By SDS-PAGE and immunoblotting, VTG was detected only in the presence of both 10â5 M of E2 and HDL. Our findings strongly support the idea that HDL correlates with vitellogenesis in eel liver.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 140, Issue 4, April 2005, Pages 543-550
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 140, Issue 4, April 2005, Pages 543-550
نویسندگان
Akiko Kumagai, Seiichi Hayashi,