کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9889671 | 1539171 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Actin gene in prawn, Macrobrachium rosenbergii: characteristics and differential tissue expression during embryonic development
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Actin gene in prawn, Macrobrachium rosenbergii: characteristics and differential tissue expression during embryonic development Actin gene in prawn, Macrobrachium rosenbergii: characteristics and differential tissue expression during embryonic development](/preview/png/9889671.png)
چکیده انگلیسی
An actin gene (named Mar-actin) from the commercial prawn, Macrobrachium rosenbergii, was isolated, sequenced and gene expression was characterized. The cDNA sequence was 1281 bp in length and contained 1131 bp open reading frame encoding 376 amino acids. The amino acid sequence deduced from the nucleotide sequence showed high identity (70.3% to 98.1%) with other known actins of various organisms, highest with that of the European flounder (98.1%). The 3â² untranslated region (3â² UTR) of the Mar-actin mRNA has a high A+U content (approximately 78%) and contains one AUUUUUA and two repeats of the AUUUA motifs, that may function in regulating mRNA decay. Northern blot analysis revealed that the Mar-actin gene was expressed predominantly in muscle tissues. Transcripts in hepatopancreas were barely detectable. Expression of the Mar-actin gene varied during embryonic development and reached the maximal level at the zoea stage. This is the first report describing the complete sequence and expression pattern of the actin gene in prawns.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 140, Issue 4, April 2005, Pages 599-605
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 140, Issue 4, April 2005, Pages 599-605
نویسندگان
Xiao-Jing Zhu, Zhong-Min Dai, Jun Liu, Wei-Jun Yang,