کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9889982 | 1539995 | 2005 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pterostilbene and 3â²-hydroxypterostilbene are effective apoptosis-inducing agents in MDR and BCR-ABL-expressing leukemia cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
FCSBCR-ABLCFU-GMFas-Lz-IETD-FMKDIOC6DISCZ-LEHD-FMKMDRSARPBSPHA-LCMDMSO - DMSOz-VAD-fmk - Z-VAD-FMKApoptosis - خزان یاختهایdimethylsulphoxide - دی متیل سولفوکسیدStructure-activity relationships - روابط ساختاری-فعالیتfoetal calf serum - سرم گوساله جنینFas-ligand - فاس لیگندPhosphate buffered saline - فسفات بافر شورleukemia - لوسمیdeath-inducing signalling complex - مجموعه ای از سیگنال های ناشی از مرگMultidrug resistance - مقاومت چند دارویی3,3′-dihexyloxacarbocyanine iodide - یدید 3،3'-dihexyloxacarbocyanine
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Pterostilbene and 3,5-hydroxypterostilbene are the natural 3,5-dimethoxy analogs of trans-resveratrol and piceatannol, two compounds which can induce apoptosis in tumor cells. In previous studies we demonstrated the importance of a 3,5-dimethoxy motif in conferring pro-apoptotic activity to stilbene based compounds so we now wanted to evaluate the ability of pterostilbene and 3,5-hydroxypterostilbene in inducing apoptosis in sensitive and resistant leukemia cells. When tested in sensitive cell lines, HL60 and HUT78, 3â²-hydroxypterostilbene was 50-97 times more potent than trans-resveratrol in inducing apoptosis, while pterostilbene appeared barely active. However, both compounds, but not trans-resveratrol and piceatannol, were able to induce apoptosis in the two Fas-ligand resistant lymphoma cell lines, HUT78B1 and HUT78B3, and the multi drug-resistant leukemia cell lines HL60-R and K562-ADR (a Bcr-Abl-expressing cell line resistant to imatinib mesylate). Of note, pterostilbene-induced apoptosis was not inhibited by the pancaspase-inhibitor Z-VAD-fmk, suggesting that this compound acts through a caspase-independent pathway. On the contrary, 3â²-hydroxypterostilbene seemed to trigger apoptosis through the intrinsic apoptotic pathway: indeed, it caused a marked disruption of the mitochondrial membrane potential ÎΨ and its apoptotic effects were inhibited by Z-VAD-fmk and the caspase-9-inhibitor Z-LEHD-fmk. Moreover, pterostilbene and 3â²-hydroxypterostilbene, when used at concentrations that elicit significant apoptotic effects in tumor cell lines, did not show any cytotoxicity in normal hemopoietic stem cells. In conclusion, our data show that pterostilbene and particularly 3â²-hydroxypterostilbene are interesting antitumor natural compounds that may be useful in the treatment of resistant hematological malignancies, including imatinib, non-responsive neoplasms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 8, August 2005, Pages 1709-1726
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 8, August 2005, Pages 1709-1726
نویسندگان
Manlio Tolomeo, Stefania Grimaudo, Antonietta Di Cristina, Marinella Roberti, Daniela Pizzirani, Maria Meli, Luisa Dusonchet, Nicola Gebbia, Vincenzo Abbadessa, Lucia Crosta, Riccardo Barucchello, Giuseppina Grisolia, Francesco Invidiata, Daniele Simoni,