کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9894306 | 1542354 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of native protein by using Synechocystis sp. PCC6803 DnaB mini-intein in Escherichia coli
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Production of native protein by using Synechocystis sp. PCC6803 DnaB mini-intein in Escherichia coli Production of native protein by using Synechocystis sp. PCC6803 DnaB mini-intein in Escherichia coli](/preview/png/9894306.png)
چکیده انگلیسی
To directly express native recombinant proteins in Escherichia coli, a new expression vector pSB was constructed using Ssp DnaB mini-intein. Using the vector, native proteins could be produced with the help of C-terminal self-cleavage of the intein. In this study, we cloned hIFNα-4 gene into pSB and used E. coli strain Origami B (DE3) as the host. Expression experiments were carried out both in Shake flasks and a 5 L bioreactor. The results indicated hIFNα-4 could be expressed in the form of soluble protein with correct folding in E. coli. The maximal hIFNα-4 content was 21.7% of total protein, and the antiviral activity of the protein was 1.2 Ã 108 IU mgâ1. Overall, good effects were achieved with this system. This intein-mediated protein expression system opens up a useful method for production of native recombinant protein in E. coli.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 40, Issue 2, April 2005, Pages 340-345
Journal: Protein Expression and Purification - Volume 40, Issue 2, April 2005, Pages 340-345
نویسندگان
Shou-Sheng Yan, Juan Yan, Gang Shi, Qi Xu, Shou-Chun Chen, Yu-Wang Tian,