کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9894363 | 1542455 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Angiotensin II AT1 receptor mutants expressed in CHO cells caused morphological change and inhibition of cell growth
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
To assess the importance of the leucine residues in positions 262 and 265 of the angiotensin AT1 receptor for signaling pathways and receptor expression and regulation, we compared the properties of CHO cells transfected with the wild type or the L262D or L265D receptor point mutants. It was found that the two mutants significantly increased the basal intracellular cyclic AMP (cAMP) formation in an agonist-independent mode. The morphology transformation of CHO cells was correlated with the increased cAMP formation, since forskolin, a direct activator of adenylate cyclase mimicked this effect on WT-expressing CHO cells. DNA synthesis was found to be inhibited in these cell lines, indicating that cAMP may also have determined the inhibitory effect on cell growth, in addition to the cell transformation from a tumorigenic to a non-tumorigenic phenotype. However a role for an increased Ca2+ influx induced by the mutants in non-stimulated cells cannot be ruled out since this ion also was shown to cause transformed cells to regain the morphology and growth regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Peptides - Volume 131, Issues 1â3, 15 November 2005, Pages 18-22
Journal: Regulatory Peptides - Volume 131, Issues 1â3, 15 November 2005, Pages 18-22
نویسندگان
Silvana A.A. Correa, Nelson A.S. Pacheco, Claudio M. Costa-Neto, Laerte Oliveira, João B. Pesquero, Sang W. Han, Antonio C.M. Paiva, Suma I. Shimuta,