کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9894452 | 1542459 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protein kinase C-mediated inhibition of renal Ca2+ ATPase by physiological concentrations of angiotensin II is reversed by AT1- and AT2-receptor antagonists
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کلمات کلیدی
tPAPMSFPMCAPLCPKCMe2SOPhosphorylationsAngiotensin II - آنژیوتانسین دوrenal transport - انتقال کلیهAng II - دومDAG - روزSaralasin - سارالوزینplasma membrane Ca2+ ATPase - غشای پلاسما Ca2 + ATPasephospholipase C - فسفولیپاز CProtein kinase C - پروتئین کیناز سیAngiotensin II receptors - گیرنده های آنژیوتانسین II
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Protein kinase C-mediated inhibition of renal Ca2+ ATPase by physiological concentrations of angiotensin II is reversed by AT1- and AT2-receptor antagonists Protein kinase C-mediated inhibition of renal Ca2+ ATPase by physiological concentrations of angiotensin II is reversed by AT1- and AT2-receptor antagonists](/preview/png/9894452.png)
چکیده انگلیسی
Angiotensin II (Ang II) increases the cytosolic Ca2+ concentration in different cell types. In this study, we investigate the effect of Ang II on the Ca2+ ATPase of purified basolateral membranes of kidney proximal tubules. This enzyme pumps Ca2+ out of the cytosol in a reaction coupled to ATP hydrolysis, and it is responsible for the fine-tuned regulation of cytosolic Ca2+ activity. Ca2+-ATPase activity is inhibited by picomolar concentrations of Ang II, with maximal inhibition being attained at â50% of the control values. The presence of raising concentrations (10â11 to 10â7 M) of losartan (an AT1-receptor antagonist) or PD123319 (an AT2-receptor antagonist) gradually reverts inhibition by Ang II. Both the phospholipase C (PLC) inhibitor U-73122 (10â6 M) and the inhibitor of protein kinase C (PKC) staurosporine (10â7 M) prevent inhibition of the Ca2+ pump by Ang II. Incubation of the previously isolated membranes with a PKC activator-the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (10â8 M)-mimics the inhibition found with Ang II, and the effects of the compounds are not additive. Taken as a whole, these results indicate the Ang II inhibits Ca2+-ATPase by activation of a PKC system present in primed state in these membranes after binding of the hormone to losartan- and PD123319-sensitive receptors coupled to a PLC. Therefore, inhibition of the basolateral membrane Ca2+-ATPase by kinase-mediated phosphorylation appears to be one of the pathways by which Ang II promotes an increase in the cytosolic Ca2+ concentration of proximal tubule cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Peptides - Volume 127, Issues 1â3, 15 April 2005, Pages 151-157
Journal: Regulatory Peptides - Volume 127, Issues 1â3, 15 April 2005, Pages 151-157
نویسندگان
Iranaia Assunção-Miranda, Adilson L. Guilherme, Clédson Reis-Silva, Glória Costa-Sarmento, Mécia M. Oliveira, Adalberto Vieyra,