کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902216 | 1545794 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
IgG subclass-independent improvement of antibody-dependent cellular cytotoxicity by fucose removal from Asn297-linked oligosaccharides
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کلمات کلیدی
ADCCIgGCDCFucoseFcγRantibody-dependent cellular cytotoxicity - آنتی بادی-وابسته به سمیت سلولی سلولیCho - برایChinese Hamster Ovary - تخمدان هامستر چینیELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاcomplement-dependent cytotoxicity - سمیت سلولی وابسته به مکملFc gamma receptor - گیرنده گاما Fc
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Fucose depletion from oligosaccharides of human IgG1-type antibodies results in a great enhancement of antibody-dependent cellular cytotoxicity (ADCC). The aim of this study was to clarify the effect of fucose removal on effector functions of all human IgG subclasses. A panel of anti-CD20 chimeric antibodies having a matched set of human heavy chain subclasses with different fucose contents in their oligosaccharides was constructed using wild-type and fucosyltransferase-knockout Chinese hamster ovary cells as host cells. As found previously for IgG1, fucose-negative variant of IgG2, IgG3, and IgG4 exhibited enhanced ADCC and FcγRIIIa binding compared with their highly fucosylated counterparts. In contrast, fucose removal did not affect complement-dependent cytotoxicity (CDC) of any IgGs. Consequently, fucose removal from IgG2 and IgG4 resulted in a unique effector function profile; they had potent ADCC and no CDC. In conclusion fucose depletion can provide a panel of IgGs with enhanced ADCC without an impact on other inherent properties specific for each IgG subclass, such as CDC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 306, Issues 1â2, 30 November 2005, Pages 151-160
Journal: Journal of Immunological Methods - Volume 306, Issues 1â2, 30 November 2005, Pages 151-160
نویسندگان
Rinpei Niwa, Akito Natsume, Aya Uehara, Masako Wakitani, Shigeru Iida, Kazuhisa Uchida, Mitsuo Satoh, Kenya Shitara,