کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902221 | 1545794 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluorescence based oxygen uptake analysis in the study of metabolic responses to apoptosis induction
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کلمات کلیدی
zVADfmkFITCJC-1ΔΨm3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideMTT - MTTROS - ROSApoptosis - خزان یاختهایelectron transport chain - زنجیره انتقال الکترونPhosphatidylserine - فسفاتیدیلسرینcaspase activity - فعالیت caspasefluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتFluorescence - فلورسنسMitochondria - میتوکندریاETc - و غیرهMitochondrial membrane potential - پتانسیل غشای میتوکندریPropidium iodide - پروتئین یدیدReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
Mitochondrial activity has been shown to be centrally involved in the progression of apoptosis. The electron transport chain is a major player in this process and oxygen uptake analysis provides detailed information on its activity. Here we examined the ability of a fluorescence based oxygen uptake assay to inform on cellular responses to apoptosis induction. HL60 cells treated with camptothecin and UV light were used as a model and the ability of the assay to detect dose and time dependent decreases in respiratory activity analysed. The data obtained were compared to more specific markers of apoptosis including annexin V binding, and caspase-3 activity. Reductions in oxygen uptake rates were seen at lower doses than increases in annexin V binding or mitochondrial membrane potential depolarisation. These reductions were observed earlier than detectable caspase-3 activity and were unaffected by pre-treatment with the caspase-3 inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoro-methylketone (zVADfmk).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 306, Issues 1â2, 30 November 2005, Pages 193-201
Journal: Journal of Immunological Methods - Volume 306, Issues 1â2, 30 November 2005, Pages 193-201
نویسندگان
James Hynes, Tomás C. O'Riordan, James Curtin, Thomas G. Cotter, Dmitri B. Papkovsky,