کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9902254 1545796 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential reactivity of anti-primate and anti-human secondary antibodies against human and monkey immunoglobulins: Implications for determining the sensitivity of immunogenicity assays
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Differential reactivity of anti-primate and anti-human secondary antibodies against human and monkey immunoglobulins: Implications for determining the sensitivity of immunogenicity assays
چکیده انگلیسی
Development of immunogenicity assays for assessment of human antibodies to therapeutic proteins requires a quantitative determination of assay sensitivity. In the absence of true human positive controls, this is usually accomplished by utilizing affinity-purified antibodies from non-human primates or monoclonal antibodies. In the former case, it is generally considered that non-human primate antibodies will be recognized equally to human antibodies by secondary anti-human immunoglobulin reagents used in immunogenicity assays. We present results here demonstrating that this is not the case. In reality, anti-human immunoglobulin secondary antibodies do not recognize primate immunoglobulins as well as human immunoglobulins. As a result, the use of affinity purified primate antibodies to determine the sensitivity of an immunogenicity assay will likely result in the true sensitivity of the assay being underestimated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 303, Issues 1–2, August 2005, Pages 76-80
نویسندگان
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