کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9908676 | 1547708 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Collapsin response mediator protein-4 regulates F-actin bundling
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کلمات کلیدی
ADFICCLatrunculin AGSK-3CRMPCytochalasin BArp2/3 - Arp2 / 3Immunocytochemistry - ایمونوسیتوشیمیactin depolymerizing factor - عامل تخلیه کننده اکتینWestern blotting - وسترن بلاتینگLatA - وصلهcollapsin response mediator protein - پروتئین متقابل پاسخ کلاپسینglycogen synthase kinase 3 - گلیکوزین سنتاز کیناز 3
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Collapsin response mediator proteins (CRMPs) form a family of cytosolic phosphoproteins which are involved in the signal transduction of semaphorin 3A leading to growth cone collapse. These proteins interact with a variety of cytosolic proteins including tubulin heterodimers. Here, we show that CRMP-4 co-localizes with F-actin in regular rib-like structures within lamellipodia of B35 neuroblastoma cells. Furthermore, depolymerization of actin fibers changed the distribution of GFP-CRMP-4 in vivo. In vitro, recombinant CRMP-4 formed homo-oligomers, bound to F-actin and organized F-actin into tight bundles. Both oligomerization and F-actin bundling depended on the C-terminal part of CRMP-4. The stoichiometry of actin and CRMP-4 in bundles was approximately 1:1 and the apparent equilibrium constant of the microfilament-CRMP-4 interaction was estimated from bundling assays as K(app) = 730 mMâ1. CRMP-4 was abundant in the cytosol of B35 neuroblastoma cells and its concentration was measured as â1.7 μM. Overexpression of CRMP-4 inhibited the migration of B35 neuroblastoma cells, while knockdown of CRMP-4 enhanced cell migration and disturbed rib-like actin-structures in lamellipodia. Taken together, our data indicate that CRMP-4 promotes bundling of F-actin in vitro, that it is an important component of rib-like actin bundles in lamellipodia in vivo and that it functionally regulates the actin cytoskeleton in motile cells. These findings suggest a specific regulatory role of CRMP-4 towards the actin cytoskeleton which may by be relevant for growth cone collapse.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 310, Issue 2, 1 November 2005, Pages 434-444
Journal: Experimental Cell Research - Volume 310, Issue 2, 1 November 2005, Pages 434-444
نویسندگان
Volker Rosslenbroich, Lingsong Dai, Stephan L. Baader, Angelika A. Noegel, Volkmar Gieselmann, Joachim Kappler,