کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9918720 1557555 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Calorimetric study of bovine serum albumin dilution and adsorption onto polystyrene particles
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی علوم دارویی
پیش نمایش صفحه اول مقاله
Calorimetric study of bovine serum albumin dilution and adsorption onto polystyrene particles
چکیده انگلیسی
Titration calorimetry was used to investigate the interaction between a model antigen, bovine serum albumin (BSA), and a model particulate carrier, polystyrene (PS). The binding enthalpy was much higher than reported in the literature for a similar system and did not display a sigmoidal binding curve. These experiments may have accessed low coverage surface sites due to the irreversible nature of protein binding and stepwise titration. An important correction is the heat of dilution of the protein solution. Two regimes were observed: at low concentrations of BSA (below ca. 0.3% (w/v)) an exothermic dilution enthalpy of ca. −100 mJ mg−1 was determined, whereas at higher concentrations of BSA values of ca. −20 mJ mg−1 were obtained. Solution rheological data also showed a change at 0.3% (w/v) BSA, so we hypothesise that the fraction of the BSA as monomers, dimers and polymers in solution changes at approximately 0.3% (w/v).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Pharmaceutics - Volume 298, Issue 2, 25 July 2005, Pages 333-338
نویسندگان
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