کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9918724 | 1557555 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Release of DNA from dendriplexes encapsulated in PLGA nanoparticles
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موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
علوم دارویی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Release of DNA from dendriplexes encapsulated in PLGA nanoparticles Release of DNA from dendriplexes encapsulated in PLGA nanoparticles](/preview/png/9918724.png)
چکیده انگلیسی
Biodegradable PLGA particles of less than 1 μm can encapsulate DNA and DNA-dendron complexes (dendriplexes) providing sustained DNA release for transfecting cells in gene delivery. Two polylysine-based dendrons prepared by solid state peptide synthesis were used to condense pRedN-1 DNA (7.5 kbp), a fluorescent protein vector. The dendrons had 16 free surface amino groups attached to seven lysine groups, bound to a lipid core, one containing three C18 chains and the other a single C10 chain. Increased lipophilicity and molar charge ratios are key factors in producing compact and reproducible dendriplexes, shown by the hydrodynamic diameter which is of the order of 800 nm (p.d. > 0.5) at a 2:1 molar charge ratio, a value which decreases to around 200 nm at a 5:1 charge ratio. At lower charge ratios the dendriplexes are negative and have a zeta potential in order of â18 mV. As the ratio increases (5:1, 10:1) the complexes bear a positive potential (13 ± 2 mV). This suggests that at the 2:1 ratio the DNA is not fully condensed. The DNA was radiolabelled with 35S dCTP (deoxycytidinetriphosphate) with the removal of the un-incorporated radiolabelled nucleotides. The encapsulation efficiency of dendriplexes in PLGA particles is higher than that for uncomplexed DNA. When the results are normalised for DNA content and particle surface area, complexation of the DNA was found to decrease release rate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Pharmaceutics - Volume 298, Issue 2, 25 July 2005, Pages 354-360
Journal: International Journal of Pharmaceutics - Volume 298, Issue 2, 25 July 2005, Pages 354-360
نویسندگان
Suzie Ribeiro, Nasir Hussain, Alexander T. Florence,