کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9955078 | 1562692 | 2018 | 48 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Using bisphenol A and its analogs to address the feasibility and usefulness of the CALUX-PPARγ assay to identify chemicals with obesogenic potential
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کلمات کلیدی
tetrachlorobisphenol ABPSBPFBPAobesogensPC10MEHPRGZTCBPARLUBFDGEHPTEbisphenol F diglycidyl etherU2OSGW9662MXC15dPGJ2TBTtetrabromobisphenol ADcfTBBPATributyltinBDE-47IC50EC50DEHPBBzP15-deoxy-Δ12,14-Prostaglandin J2 - 15-deoxy-Δ12،14-پروستاگلاندین J2TZDs - TZD هاBADGE - بدBisphenols - بیسفنولbisphenol A diglycidyl ether - بیسفنول A diglycidyl etherBisphenol F - بیسفنول Fbisphenol S - بیسفنول SBisphenol A - بیسفنول ای، بیسفنول Athiazolidinediones - تیازولیدیدونهاDi(2-ethylhexyl)phthalate - دی (2-اتیل هگزیل) فتالاتDiclofenac - دیکلوفناکrosiglitazone - روزیگلیتازونIn vitro screening - غربالگری درون in vitrohalf-maximal effective concentration - غلظت موثر نیمه حداکثرMetabolism - متابولیسم Methoxychlor - متوکسی کلرMono(2-ethylhexyl)phthalate - مونو (2-اتیل هگزیل) فتالاتhalf-maximal inhibitory concentration - نیمه حداکثر غلظت مهاریrelative light units - واحدهای نسبی نورCALUX - کلوکس
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Environmental chemical exposures have been implicated in the obesity epidemic as potential mis-regulators of a variety of metabolic pathways. As agonism of the peroxisome proliferator-activated nuclear hormone receptor γ (PPARγ) is one of the suspected mechanisms involved, a PPARγ screening assay may have relevance for the biodetection of such effects of environmental chemicals. To test this hypothesis, we established the PPARγ2-CALUX® assay in-house and tested it against a number of known and suspected PPARγ modulators. Furthermore, we added a rat liver S9 metabolizing system to the protocol to introduce metabolic competence to the assay. Our results confirmed the responsiveness of the cell line to the known PPARγ agonists and antagonists: rosiglitazone, tributyltin, 15-deoxy-Î12,14-prostaglandin J2, GW9662 and diclofenac. These data are in agreement with previous studies in various models. Seven bisphenol analogs tested induced little to no agonist activity, but all demonstrated antagonistic properties. These findings were contrary to both our assumptions and literature reports. Addition of the S9-metabolizing system to each of these tests did not alter any of the measured activities. Taken together, it seems probable that there are additional obesogenic effects of these chemicals which would not be detected by this assay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 53, December 2018, Pages 208-221
Journal: Toxicology in Vitro - Volume 53, December 2018, Pages 208-221
نویسندگان
Camille Dusserre, Julie Mollergues, Elena Lo Piparo, Martin Smieško, Maricel Marin-Kuan, Benoit Schilter, Karma Fussell,