کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9955308 | 1569087 | 2018 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oral exposure to the free amino acid glycine inhibits the acute allergic response in a model of cow's milk allergy in mice
ترجمه فارسی عنوان
مواجهه دهانی با گلیسین آمینو اسید آزاد باعث مهار پاسخ حساسیتی حاد در یک مدل حساسیت شیر به گاو در موش می شود
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کلمات کلیدی
CMAGlyRT helper type 2RBLTh2mMCP-1mouse mast cell protease-1cow's milk allergy - آلرژی شیر گاوFood allergy - آلرژی غذاییamino acid - آمینو اسیدimmunoglobulin - ایمونوگلوبولینbasophils - بازوفیل هاDendritic cell - سلول دندریتیکrat basophilic leukemia cells - سلول های لوسمی بازوفیل موشIntestinal epithelial cells - سلولهای اپیتلیال رودهMast cells - ماست سل هاIEC - کمیسیون مستقل انتخاباتglycine receptor - گیرنده گلیسین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
چکیده انگلیسی
The conditionally essential amino acid glycine functions as inhibitory neurotransmitter in the mammalian central nervous system. Moreover, it has been shown to act as an anti-inflammatory compound in animal models of ischemic perfusion, post-operative inflammation, periodontal disease, arthritis and obesity. Glycine acts by binding to a glycine-gated chloride channel, which has been demonstrated on neurons and immune cells, including macrophages, polymorphonuclear neutrophils and lymphocytes. The present study aims to evaluate the effect of glycine on allergy development in a cow's milk allergy model. To this end, C3H/HeOuJ female mice were supplemented with glycine by oral gavage (50 or 100 mg/mouse) 4 hours prior to sensitization with cow's milk whey protein, using cholera toxin as adjuvant. Acute allergic skin responses and anaphylaxis were assessed after intradermal allergen challenge in the ears. Mouse mast cell protease-1 (mMCP-1) and whey specific IgE levels were detected in blood collected 30 minutes after an oral allergen challenge. Jejunum was dissected and evaluated for the presence of mMCP-1-positive cells by immunohistochemistry. Intake of glycine significantly inhibited allergy development in a concentration dependent manner as indicated by a reduction in; acute allergic skin response, anaphylaxis, serum mMCP-1 and serum levels of whey specific IgE. In addition, in-vitro experiments using rat basophilic leukemia cells (RBL), showed that free glycine inhibited cytokine release but not cellular degranulation. These findings support the hypothesis that the onset of cow's milk allergy is prevented by the oral intake of the amino acid glycine. An adequate intake of glycine might be important in the improvement of tolerance against whey allergy or protection against (whey-induced) allergy development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nutrition Research - Volume 58, October 2018, Pages 95-105
Journal: Nutrition Research - Volume 58, October 2018, Pages 95-105
نویسندگان
Jeroen van Bergenhenegouwen, Saskia Braber, Reinilde Loonstra, Nicole Buurman, Lieke Rutten, Karen Knipping, Paul J. Savelkoul, Lucien F. Harthoorn, Frode L. Jahnsen, Johan Garssen, Anita Hartog,