کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9989622 | 1580761 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
17β-estradiol rescues spinal motoneurons from AMPA-induced toxicity: A role for glial cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
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چکیده انگلیسی
The ability of astrocytes to mediate 17β-estradiol neuroprotection of spinal motoneurons challenged with AMPA has been evaluated in a co-culture system in which pure motoneurons were pulsed with 20 μM AMPA and then transferred onto an astrocyte layer pretreated for 24 h with 10 nM 17β-estradiol. Under these conditions, AMPA toxicity was reverted, an effect that was likely related to increased production and release of GDNF, as shown by RT-PCR, Western blot analysis and ELISA assay. In addition, treatment with GDNF during the 24 h that followed the AMPA pulse produced a similar neuroprotective effect, whereas addition of a neutralizing anti-GDNF antibody prevented neuroprotection. These data suggest a role for astrocytes in the neuroprotective effect of 17β-estradiol against spinal motoneuron death and find strong support in the marked up-regulation of estrogen receptor α found in spinal astrocytes of amyotrophic lateral sclerosis patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 20, Issue 2, November 2005, Pages 461-470
Journal: Neurobiology of Disease - Volume 20, Issue 2, November 2005, Pages 461-470
نویسندگان
Paola Platania, Giovanna Seminara, Eleonora Aronica, Dirk Troost, Maria Vincenza Catania, Maria Angela Sortino,