کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9989684 | 1580763 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synaptophysin enhances the neuroprotection of VMAT2 in MPP+-induced toxicity in MN9D cells
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کلمات کلیدی
MN9DeGFPTBZα-methyl-p-tyrosineDAergicDATaMPTVMAT2Dopamine transporter - انتقال دهنده دوپامینParkinson's disease - بیماری پارکینسونTetrabenazine - تتراپرانازینtyrosine hydroxylase - تیروزین هیدروکسیلازDopamine - دوپامینDopaminergic - دوپامینرژیکSynaptophysin - سیناپتوفیزینSyn - سینتpostnuclear supernatant - فلوئور اتانولNeuroprotection - محافظت نورونی یا محافظت از عصبvesicular monoamine transporter 2 - مونوآمین حامل 2enhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استPNS - کارمندان دولت
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The use of the potent neurotoxin MPTP in producing a model for Parkinson's disease (PD) has allowed us to dissect the cellular processes responsible for both selective neuronal vulnerability and neuroprotection in idiopathic PD. It has been suggested that vesicular monoamine transporters (VMATs) play a critical neuroprotective role in MPP+ toxicity. However, little is known about how this detoxificative sequestration in dopaminergic (DAergic) neurons is regulated at the molecular and cellular levels. Using the DAergic cell line MN9D as an in vitro model, we found that overexpression of VMAT2 (a neuronal isoform of VMATs) protects the transformants from MPP+-induced toxicity, consistent with the previous work on fibroblastic CHO cells. We further found that the MN9D cells displayed lower expression levels of secretory vesicle proteins such as synaptophysin. Overexpression of synaptophysin in MN9D cells can significantly increase the resistance of the transformants to MPP+ toxicity. The co-expression of VMAT2 and synaptophysin has shown synergistic protection for the transformants, suggesting a role of synaptophysin in the biogenesis of secretory vesicles and in influencing the targeting of VMAT2 to these vesicles. Our work indicates that both the expression level of VMAT2 and capacity of vesicular packaging of DA are important in protecting DAergic cells from MPP+ toxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 19, Issue 3, August 2005, Pages 419-426
Journal: Neurobiology of Disease - Volume 19, Issue 3, August 2005, Pages 419-426
نویسندگان
Carol X.-Q. Chen, Steven Y. Huang, Limei Zhang, Yong-Jian Liu,