کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9996684 | 1583421 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of agonist-induced Ca2+ responses in rat submandibular acini and ducts
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کلمات کلیدی
HEPESmRNAPROPGAPDHCCHRT-PCREPIIP32-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid - 2- [4- (2-hydroxyethyl) -1-piperazinyl] ethanesulfonic acid[Ca2+]i - [Ca2 +] iacini - آکینیEpinephrine - اپی نفرینISO - ایزوisoproterenol - ایزوپروترنولIntracellular Ca2+ - داخل سلولی Ca2 +Submandibular glands - غدد submandibularintracellular calcium concentration - غلظت کلسیم داخل سلولیSubstance P - ماده PReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایPropranolol - پروپرانولولPhentolamine - پنتولامینCarbachol - کارباکولducts - کانال هاglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازMembrane receptor - گیرنده غشایی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Comparison of agonist-induced Ca2+ responses in rat submandibular acini and ducts Comparison of agonist-induced Ca2+ responses in rat submandibular acini and ducts](/preview/png/9996684.png)
چکیده انگلیسی
Changes in intracellular Ca2+ concentration ([Ca2+]i) induced by agonists were simultaneously monitored in rat submandibular acini and ducts using a Ca2+ imaging system. Substance P (SP) elicited marked increases in [Ca2+]i in acini but not in ducts. Carbachol (CCh) increased [Ca2+]i in both acini and ducts, but the maximal level was higher in acini than in ducts. In contrast, epinephrine (Epi) also induced an increase in [Ca2+]i in acini and ducts, but to a greater extent in ducts than in acini. Isoproterenol (ISO) caused a small but significant increase in [Ca2+]i in ducts but not acini. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis using total RNA extracted from highly purified acinar and ductal cells showed that substance P receptor mRNA was present in acini at higher levels than in ducts. In contrast, α1a-adrenoceptor mRNA was more strongly expressed in ducts than in acini. The muscarinic receptors (M3 and M5) and β-adrenoceptors (β1 and β2) were expressed at equivalent levels in both cell types. These results confirm that acini and ducts exhibit significant differences in agonist-induced Ca2+ responses. Furthermore, substance P- and epinephrine-induced Ca2+ responses were consistent with receptor mRNA expression in acini and ducts, but carbachol- and isoproterenol-induced [Ca2+]i increases were not.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 50, Issue 6, June 2005, Pages 585-592
Journal: Archives of Oral Biology - Volume 50, Issue 6, June 2005, Pages 585-592
نویسندگان
Akihiro Nezu, Takao Morita, Akihiko Tanimura, Yosuke Tojyo,