A quick and reliable method for monitoring gene expression in actinomycetes
Keywords: Amycolatopsis balhimycina; Balhimycin; egfp; Fluorescence
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Interactions between the budding yeast IQGAP homologue Iqg1p and its targets revealed by a split-EGFP bimolecular fluorescence complementation assay
Keywords: Cytoskeleton; Protein-fragment complementation assay (PCA); Calmodulin; Myosin light chain; CDC42; Enhanced green fluorescent protein (EGFP); BiFC; bimolecular fluorescence complementation assay; EGFP; enhanced green fluorescent protein; EGFP-C158; EGFP f
Calmodulin kinase II inhibition disrupts cardiomyopathic effects of enhanced green fluorescent protein
Keywords: Calmodulin kinase II; Cardiomyopathy; eGFP
Leukotriene C4 synthase promoter driven expression of GFP reveals cell specificity
Keywords: Leukotriene; Leukotriene C4 synthase; Expression; GFP; TPA; Promoter; Retinoic acid; DMSO; AP-2; activating protein 2; C/EBP; CCAAT/enhancer binding protein; eGFP; enhanced green fluorescent protein; HEK293/T; human embryonic kidney 293T cell line; HL-60;
Bilayer microfluidic chip for diffusion-controlled activation of yeast species
Keywords: eGFP; Saccharomyces cerevisiae; Microfluidics; Microenvironments; Fluorescence microscopy
Tubulin superfamily genes in Tribolium castaneum and the use of a Tubulin promoter to drive transgene expression
Keywords: Tribolium; Tubulin; Promoter; PiggyBac; EGFP; Vermilion; Transgene
Eukaryotic expression and secretion of EGFP-labeled annexin A5
Keywords: Apoptosis; Annexin A5; EGFP; Eukaryotic expression; Secretion
Effect of ABCG2 on cytotoxicity of platinum drugs: Interference of EGFP
Keywords: Platinum cytostatics; ABCG2; MTT assay; Neutral red assay; Cytotoxicity; Apoptosis; EGFP
Generation and characterization of an Npro-disrupted marker bovine viral diarrhea virus derived from a BAC cDNA
Keywords: BVDV; EGFP; FMDV 2A protease; Transplacental infection; Persistent infection; BAC cDNA
NUMBL interacts with TAB2 and inhibits TNFα and IL-1β-induced NF-κB activation
Keywords: NF-κB; nuclear factor kappa B; IL-1β; interleukin-1β; TNF; tumor necrosis factor; NUMBL; homologous protein of numb like; TAK1; TGF-β activated kinase1; TAB2; TAK1-binding protein 2; TRAF6; TNF receptor-associated factor 6; TRAF2; TNF receptor-associa
Bovine viral diarrhea virus is a suitable viral vector for stable expression of heterologous gene when inserted in between Npro and C genes
Keywords: BVDV; FMDV 2Apro; EGFP; Viral vector; Stable expression
Construction of a new plasmid for surface display on cells of Yarrowia lipolytica
Keywords: Surface display vectors; EGFP; Haemolysin; Yarrowia lipolytica; GPI-anchor
Overexpression of SPINDLIN1 induces cellular senescence, multinucleation and apoptosis
Keywords: 7-AAD; 7-Aminoactinomycin D; DAPI; 4', 6-diamidino-2-phenylindole; EGFP; Enhanced green fluorescent protein; PBS; Phosphate-buffered saline; SA-β-gal activity; Senescence-associated β-galactosidase.; Apoptosis; Cell death; Senescence; SPINDLIN1;
Isolation and characterization of mutant animal cell line defective in alkyl-dihydroxyacetonephosphate synthase: Localization and transport of plasmalogens to post-Golgi compartments
Keywords: AOx; acyl-CoA oxidase; ADAPS; alkyl-dihydroxyacetonephosphate synthase; BFA; brefeldin A; β-MCD; β-methyl cyclodextrin; CHO; Chinese hamster ovary; DHAPAT; dihydroxyacetonephosphate acyltransferase; DRM; detergent-resistant membranes; EGFP; “enhancedâ
Maintenance of systemic immune functions prevents accelerated presbycusis
Keywords: EGFP; enhanced green fluorescent protein; [EGFPâSAMP1] mice; SAMP1 mice transplanted with BMCs from EGFP mice; Con A; concanavalin A; SGCs; spiral ganglion cells; BMT; bone marrow transplantation; ABR; auditory brainstem response; HSCs; hematopoietic st
Enhanced green fluorescent protein-expressing human mesenchymal stem cells retain neural marker expression
Keywords: Culture; Neural differentiation; Stem cells; EGFP
Dopamine D1 receptor-mediated aggregation of N-terminal fragments of mutant huntingtin and cell death in a neuroblastoma cell line
Keywords: Huntington's disease; G-protein-coupled receptors; cell survival; dopamine receptors; cAMP; 3â²-5â²-cyclic AMP; CREB; 3â²-5â²-cyclic AMP response element-binding; DMEM; Dulbecco's modified eagle's medium; EGFP; enhanced green fluorescent protein; GAPD
Posttranscriptional regulation of IL-13 in T cells: Role of the RNA-binding protein HuR
Keywords: Asthma; inflammation; mRNA turnover; posttranscriptional gene regulation; RNA-binding proteins; IL-13; TH2 cytokines; T cells; ARE; Adenylate-uridylate-rich element; CT; Cycle threshold; DTT; Dithiothreitol; EGFP; Enhanced green fluorescent protein; GAP
Constitutive CXCL12 Expression Induces Anoikis in Colorectal Carcinoma Cells
Keywords: ECM; extracellular matrix; eGFP; enhanced green fluorescent protein; ERK1/2; extracellular-regulated kinase-1/2; FAK; focal adhesion kinase; MEK1; mitogen-activated protein kinase kinase 1; poly-HEMA; polyhydroxyethylmethacrylate; SCID; severe combined im
Isolation of embryonic stem cells from enhanced green fluorescent protein-transgenic mouse and their survival in the cochlea after allotransplantation
Keywords: Allotransplantation; cochlea; EGFP; ES cells; transgenic
Estrogen receptor beta in the brain: From form to function
Keywords: 3β-diol; 5α-androstane-3β,17β-diol; αERKO; estrogen receptor alpha knockout; βERKO; estrogen receptor beta knockout; AA; amino acid; ACTH; adrenocorticotropin hormone; AP-1; activator protein complex-1; AR; androgen receptor; AVP; arginine vasopress
Evidence for a detrimental role of nitric oxide synthesized by endothelial nitric oxide synthase after peripheral nerve injury
Keywords: eNOS; hypoglossal nerve; adenoviral vectors; peripheral neuropathy; Ad; adenovirus; AG; aminoguanidine; CMAP; compound muscle action potential; d-NAME; NÏ-nitro-d-arginine methyl ester; eGFP; enhanced green fluorescent protein; eNOS; endothelial isoform
Use of a new polyclonal antibody to study the distribution and glycosylation of the sodium-coupled bicarbonate transporter NCBE in rodent brain
Keywords: slc4a10; CNS; brain; oocytes; glycosylation; aa; amino acid; AE; anion exchanger; AP; affinity purification; AQP1; aquaporin 1; CB; cerebellum; Ct; C terminus; CX; cerebral cortex; EGFP; enhanced green fluorescent protein; E18; embryonic day 18; GST; glut
Polysialylated NCAM Represses E-Cadherin-Mediated Cell-Cell Adhesion in Pancreatic Tumor Cells
Keywords: EGFP; enhanced green fluorescence protein; endo-N; endoneuraminidase-N; NCAM; neural cell adhesion molecule; PSA; polysialic acid; PSA-NCAM; polysialylated neural cell adhesion molecule;
Localization, Trafficking, and Significance for Acid Secretion of Parietal Cell Kir4.1 and KCNQ1 K+ Channels
Keywords: AR; apical membrane resting state; AS; stimulus-associated membranes; [14C]AP; [14C]dimethylamine-aminopyrine; carbachol; (2-hydroxyethyl)trimethylammonium chloride carbamate; BLM; basolateral membrane fractions; EGFP; enhanced green fluorescent protein;
Analysis of innate immune signal transduction with autocatalytic expression vectors
Keywords: IL-1; interleukin-1; IL8; interleukin-8; TLR; toll-like receptor; IL-1RI; type 1 interleukin-1 receptor; TIR; Toll/interleukin-1 receptor; miR; micro-ribonucleic acid; LPS; lipopolysaccharide: NF-κB, Nuclear Factor kappa B(see also RelA); RelA; reticuloe
Characterization of a nervous system-specific promoter for growth-associated protein 43 gene in Medaka (Oryzias latipes)
Keywords: GAP-43; growth-associated protein 43; Tbp; TATA-binding protein; eGFP; enhanced green fluorescent protein; WISH; whole-mount in situ hybridization; hpf; hour(s) post fertilization; dpf; day(s) post fertilization; dpt; day(s) post transfection; PKC; protei
Nuclear localization of enhanced green fluorescent protein homomultimers
Keywords: EGFP; Dimer; Trimer; Tetramer; Hexamer; Subcellular localization; In-gel fluorescence; Native electrophoresis
Single cell transfection using plasmid decorated AFM probes
Keywords: Individual cell transfection; Atomic force microscopy; HEK293; EGFP; Membrane penetration
A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells
Keywords: AcMNPV; Autographa califonica multicapsid nuclear polyhedrovirus; EGFP; enhanced green fluorescence protein; MOI; multiplicity of infection; pfu; plaque forming unit; Baculovirus; Transduction; B-lymphocyte; Surface display; Epstein-Barr virus;
Mitochondrial import of Omi: The definitive role of the putative transmembrane region and multiple processing sites in the amino-terminal segment
Keywords: AIF; apoptosis-inducing factor; EGFP; enhanced-green fluorescence protein; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; IM; inner membrane; IMS; intermembrane space; MPP; mitochondrial processing peptidase; MTS; mitochondrial targeting sequence; PDZ;
Enhancement of Exogenous Gene Expression by the Artificial Transcription Factor in Chinese Hamster Ovary Cells
Keywords: artificial transcription factors; CHO cells; EGFP; t-PA; high level expression;
Peptide rescues GLUT4 recruitment, but not GLUT4 activation, in insulin resistance
Keywords: GLUT; glucose transporter; DMEM; Dulbecco's modified Eagle's medium; HA; hemagglutinin; EGFP; enhanced green fluorescent protein; GLUT4; GLUT1; Recruitment to the plasma membrane; Glucose uptake; Insulin resistance; Phosphoinositide-binding peptide;
ALS2CL, a novel ALS2-interactor, modulates ALS2-mediated endosome dynamics
Keywords: ALS2CL; ALS2 carboxy-terminal like; MNDs; motor neuron diseases; GEF; guanine-nucleotide exchange factor; RLD; RCC1-like domain; DH; Dbl homology; PH; pleckstrin homology; VPS9; vacuolar protein sorting 9; MORN; membrane occupation and recognition nexus;
Urothelial Inhibition of Transforming Growth Factor-β in a Bladder Tissue Recombination Model
Keywords: urothelium; bladder; transforming growth factors; transfection; mesoderm; ABC; avidin-biotin complex; DAB; 3,3â²-diaminobenzidine tetrahydrochloride; DNIIR; dominant negative form of type II TGF-β receptor; EGFP; enhanced green fluorescence peptide; FAC
Establishment of a newly improved detection system for NF-κB activity
Keywords: NF-κB; Transcription; Reporter system; Luciferase; EGFP
Imidazole as a catalyst for in vitro refolding of enhanced green fluorescent protein
Keywords: Protein refolding; Protein aggregation; Prolyl isomerization; EGFP; Imidazole
A universal transgene silencing approach in baculovirus–insect cell system
Keywords: RNAi; siRNA; Baculovirus; Bicistronic; Sf9; LqqIT2; Fusion; EGFP
Akyrin repeat and SOCS box containing protein 4 (Asb-4) interacts with GPS1 (CSN1) and inhibits c-Jun NH2-terminal kinase activity
Keywords: Asb-4; ankyrin repeat and SOCS box-containing protein 4; Asb-4/Îsb; Asb-4 minus its SOCS box; GPS1; G-protein pathway suppressor 1; CSN1; COP9 signalosome, subunit 1; ARC; arcuate nucleus; POMC; pro-opiomelanocortin; NPY; neuropeptide Y; QDO; quadruple d
Focal cerebral ischemia/reperfusion injury in mice induces hematopoietic prostaglandin D synthase in microglia and macrophages
Keywords: PGD2; PGDS; microglia/macrophage; inflammation; ischemic stroke; tissue repair; COX; cyclooxygenase; EGFP; enhanced green fluorescent protein; GFAP; glial fibrillary acidic protein; GST-Ï; glutathione S-transferase-pi; H-PGDS; hematopoietic prostaglandin
Presynaptic AMPA and kainate receptors increase the size of GABAergic terminals and enhance GABA release
Keywords: Cerebellum; Survival; Synapses; Interneuron; Patch-clamp; eGFP
Bi-directional Cell Trafficking Between Mother and Fetus in Mouse Placenta
Keywords: Placenta; Microchimerism; Transgenic mouse; EGFP;
RIG1 suppresses Ras activation and induces cellular apoptosis at the Golgi apparatus
Keywords: EGF; epidermal growth factor; EGFP; enhanced green fluorescent protein; ER; endoplasmic reticulum; ERK; extracellular-signal-regulated protein kinase; FBS; fetal bovine serum; JNK; c-Jun N-terminal kinase; PARP; poly(ADP-ribose) polymerase; PBS; phosphate
Enhanced expression of EGFP gene in CHSE-214 cells by an ARS element from mud loach (Misgurnus mizolepis)
Keywords: Mud loach; Matrix attachment regions (MARs); Autonomously replicating sequences (ARSs); Fish cell lines; EGFP
Quaternary structure and apical membrane sorting of the mammalian NaSi-1 sulfate transporter in renal cell lines
Keywords: NaSi-1; sodium-dependent sulfate cotransporter; EGFP; enhanced green fluorescence protein; OK; opossum kidney cells; MDCK; Madin-Darby canine kidney cells; LLC-PK1; pig kidney proximal tubular cell; BN-PAGE; blue native-polyacrylamide gel electrophoresis;
Characterisation of a bis(5â²-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster
Keywords: Apf; D. melanogaster bis(5â²-nucleosyl)-tetraphosphatase (asymmetrical); ApnA (n = 2-6); diadenosine 5â²,5â´-P1; Pn-polyphosphate; ApnGP1-(5â²-adenosyl) Pn-(5â²-guanosyl) polyphosphate; DAPI; 4â²,6-diamidino-2-phenylindole; DIPP; diphosphoinosit
Histone H1 variant, H1R is involved in DNA damage response
Keywords: BrdU; bromo-deoxy-uridine; DSB; double-stranded DNA break; EGFP; enhanced green fluorescent protein; epc; event per cell; FCS; fetal calf serum; FRAP; fluorescence recovery after photobleaching; HR; homologous recombination; IR; ionizing radiation; MMC; m
Reciprocal Targeting of Hath1 and β-Catenin by Wnt Glycogen Synthase Kinase 3β in Human Colon Cancer
Keywords: APC; adenomatous polyposis coli; EGFP; enhanced green fluorescent protein; G3PDH; glyceraldehyde-3-phosphate dehydrogenase; GSK3β; glycogen synthase kinase 3b; RIPA; radioimmunoprecipitation assay; RT-PCR; reverse-transcription polymerase chain reaction;