Improved strategies for the delivery of GFP-based Ca2+ sensors into the mitochondrial matrix
Keywords: GFP; Ca2+ probes; Mitochondrial targeting;
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Elimination of environmental sensitivity in a cameleon FRET-based calcium sensor via replacement of the acceptor with Venus
Keywords: IP3; VC6.1; YC6.1; Calcium; FRET; Sensor; GFP;
Effects of environmental stresses on the activities of the uspA, grpE and rpoS promoters of Escherichia coli O157:H7
Keywords: E. coli O157:H7; uspA; grpE; rpoS; gfp; Environmental stresses;
Analysis of Ras and Rap activation in living cells using fluorescent Ras binding domains
Keywords: Ras; Rap1; GFP; Ras binding domain (RBD); FRET;
Monitoring spatio-temporal regulation of Ras and Rho GTPases with GFP-based FRET probes
Keywords: Imaging; FRET; GFP; Small G-protein;
Zizimin2: a novel, DOCK180-related Cdc42 guanine nucleotide exchange factor expressed predominantly in lymphocytes
Keywords: GC; germinal center; BCR; B cell receptor; GEF; guanine nucleotide exchange factor; CDM; CED-5, DOCK180, Myoblast city; CZH; CDM-zizimin homology; GFP; green fluorescence protein; PNA; peanuts agglutinin; PH; pleckstrin homology; GST; glutathione S-transf
High efficiency of genetic transformation and regeneration of Artemisia annua L. via Agrobacterium tumefaciens-mediated procedure
Keywords: GFP; green fluorescent protein; NAA; α-naphthaleneacetic acid; 6-BA; 6-benzylaminopurine; As; acetosyringone; Kan; kanamycin; Cefo; cefotaxime; PCR; polymerase chain reaction; bp; base pairs; T-DNA; transfer-DNA; Artemisia annua L.; Agrobacterium tumefac
Regulation of short-distance transport of RNA and protein
Keywords: CmPP; Cucurbita maxima phloem protein; CmPSRP1; Cucurbita maxima phloem SMALL RNA BINDING PROTEIN1; GFP; green fluorescent protein; ISE1; INCREASED SIZE EXCLUSION LIMIT OF PLASMODESMATA 1; KN1; KNOTTED1; LFY; LEAFY; Me; Mouse ears; MP; movement protein; N
Time reduction and process optimization of the baculovirus expression system for more efficient recombinant protein production in insect cells
Keywords: Baculovirus; BEVS; Protein expression; GFP; Expression conditions; Insect cells; Centrifugation;
Development of new inbred transgenic strains of rats with LacZ or GFP
Keywords: Transgenic rat; LacZ; GFP; Immunogenicity; Regenerative medicine; Cellular trafficking;
Subcellular localisation and silencing of ripening-associated membrane protein (TRAMP) in tomato Lycopersicon esculentum Mill.
Keywords: TRAMP; tomato ripening-associated membrane protein; GFP; green fluorescent protein; MIP; major intrinsic protein; TIP; tonoplast intrinsic protein; PIP; plasma membrane intrinsic protein; NLM; Nodulin 26-like major intrinsic protein; N; nucleus; PM; plasm
Depletion of the AP-1 repressor JDP2 induces cell death similar to apoptosis
Keywords: Aa; amino acid; CM; Complete medium; CMV; Cytolomegavirus; CRE; cyclic AMP response element; DTT; dithiothreitol; E1A and E1B; Adenovirus 5 early region 1A and 1B; FCS; Fetal calf serum; GFP; Green fluorescent protein; HDAC3; Histone deacetylase complex 3
Overexpression of OsSIN, encoding a novel small protein, causes short internodes in Oryza sativa
Keywords: BAC; bacterial artificial chromosome; bp; base pair(s); CaMV; cauliflower mosaic virus; cDNA; DNA complementary to RNA; GA; gibberellin; GFP; green fluorescent protein; kb; kilobase(s); ORF; open reading frame; PAC; paclobutrazol; RACE; rapid amplificatio
Dynamic association of topoisomerase II to the mitotic chromosomes in live cells of Aspergillus nidulans
Keywords: Topoisomerase II; Aspergillus nidulans; Time-lapse microscopy; GFP; Mitotic chromosomes;
Cloning and expression of ligand-gated ion-channel receptor L2 in central nervous system
Keywords: Ligand-gated ion channels; In silico cloning; L2 receptor; Hippocampal neurons; GFP; HEK293 cells; Immunolocalization;
Comparison of different signal peptides for secretion of heterologous proteins in fission yeast
Keywords: Schizosaccharomyces pombe; Signal peptides; Secretory pathway; GFP; TNFα; Copy number;
Redox regulation of the transcriptional repressor Bach1
Keywords: AP-1; activator protein-1; ARE; antioxidant responsive element; Bach1; BTB and CNC homology; bZIP; basic leucine zipper; CNC; Cap'n'Collar; EMSA; electrophoretic mobility shift assay; GCS; γ-glutamylcysteine synthetase; GFP; green fluorescent protein
Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-β
Keywords: PEG; poly(ethylene glycol); FBS; fetal bovine serum; PBS; phosphate-buffered saline; aa; amino acids; LB medium; Luria-Bertani medium; GFP; green fluorescent protein; GST; glutathione S-transferase; NPC; nuclear pore complex; NLS; nuclear localization s
Macrophage colony-stimulating factor receptor induces tyrosine phosphorylation of SKAP55R adaptor and its association with actin
Keywords: M-CSF; macrophage colony-stimulating factor; SFK; Src family kinases; PI3K; phosphatidyl inositol 3â² kinase; GFP; green fluorescent protein; SKAP55; Src kinase-associated phosphoprotein of 55 kDa; SKAP55R; SKAP55-related protein; SLAP130/Fyb; SLP-76-ass
Imaging protein molecules using FRET and FLIM microscopy
Keywords: FLIM; fluorescence lifetime imaging; FRET; Förster (fluorescence) resonance energy transfer; GFP; green fluorescent protein; SBT; spectral bleed-through; TIRFM; total internal reflection fluorescence microscopy;
Solubilization, purification, and mass spectrometry analysis of the human mu-opioid receptor expressed in Pichia pastoris
Keywords: GPCR; Solubilization; Detergent; Purification; GFP; Mu-opioid receptor; Pichia pastoris;
A DELLAcate balance: the role of gibberellin in plant morphogenesis
Keywords: ABA; abscisic acid; AGL15; AGAMOUS-LIKE15; AP; APETALA; BEL; BELL; DDF1; DWARF AND DELAYED FLOWERING1; FUS3; FUSCA3; GA; gibberellin; GA20ox; GA 20-oxidase; GAI; GA-INSENSITIVE; GFP; green fluorescent protein; GID2; GA-INSENSITIVE DWARF2; KNOX; KNOTTED1-l
Hepatic potential of bone marrow stromal cells: Development of in vitro co-culture and intra-portal transplantation models
Keywords: AFP; α-fetoprotein; BDL; bile duct ligation; BMSC; bone marrow stromal cells; CCl4; carbon tetrachloride; GFP; green fluorescent protein; Bone marrow; Mesenchymal stem cells; Hepatocyte; Cirrhosis;
Measurement of bacterial adhesion-in vitro evaluation of different methods
Keywords: Adhesion; Crystal violet; DAPI; EYFP; Fluorescence; GFP; Probiotic; Radioactively labelled;
LY293111 Improves Efficacy of Gemcitabine Therapy on Pancreatic Cancer in a Fluorescent Orthotopic Model in Athymic Mice
Keywords: Pancreatic cancer; LY293111; gemcitabine; GFP; orthotopic; tumor model;
Essential role of cytoplasmic sequences for cell-surface sorting of the lectin-like oxidized LDL receptor-1 (LOX-1)
Keywords: Lectin-like oxidized LDL receptor-1; Cell-surface sorting; Integral membrane protein topology; BSA; bovine serum albumin; CHO cell; Chinese hamster ovary cell; DiI; 1,1â²-dioctadecyl-3,3,3â²,3â²-tetramethylindocarbocyanine perchlorate; GFP; green fluor
Satellite cells, myoblasts and other occasional myogenic progenitors: Possible origin, phenotypic features and role in muscle regeneration
Keywords: BMP2; bone morphogenetic protein 2; GFP; green fluorescent protein; HSC; hematopoietic stem cell; MSC; mesoderm stem cell (referred to as non hematopoietic); PKC; protein kinase C; Shh; sonic hedgehog; SP; side population; TGFβ; transforming growth facto
Functional analysis of tumor necrosis factor gene promoter from Japanese flounder, Paralichthys olivaceus, using fish cell lines
Keywords: Japanese flounder; Cell line; TNF; LPS-inducible; Promoter; GFP; Innate immunity; Cytokine; TNF, tumor necrosis factor; GFP, green fluorescence protein; LPS, lipopolysaccharide;
Mitochondrial movement and inheritance in budding yeast
Keywords: GFP; green fluorescent protein; mtDNA; mitochondrial DNA; Actin; Cytoskeleton; Organelle motility; Retention; Retrograde flow;
In silico diagnosis of inherently inhibited gene expression focusing on initial codon combinations
Keywords: DB; downstream box; DE; downstream element; DR; downstream region; DsRed; Discosoma sp. red fluorescent protein; GA; genetic algorithm; GFP; green fluorescent protein; ITI; initial translation index; DsRed; Genetic algorithm; Green fluorescent protein; Tr
Drag&Drop cloning in yeast
Keywords: aa; amino acid(s); bp; base pair(s); G418; geneticin; GFP; green fluorescent protein; GST; glutathione-S-transferase; kb; kilobase(s); kDa; kilodalton(s); kanR; kanamycin resistance; mprs; multi-purpose recombination site; myr; myristoylation; OD; optical
The plastidic DNA replication enzyme complex of Plasmodium falciparum
Keywords: GFP; green fluorescent protein; PATS; prediction of apicoplast targeting sequence; RT-PCR; reverse transcriptase-polymerase chain reaction; MEGA; molecular evolution genetic analysis; NJ; neighbour joining; MP; maximum parsimony; ME; minimum evolution; UP
Internalin-expressing Lactococcus lactis is able to invade small intestine of guinea pigs and deliver DNA into mammalian epithelial cells
Keywords: Lactococcus lactis; Listeria monocytogenes; Internalin A; DNA delivery; GFP; green fluorescent protein; InlA; internalin; LAB; lactic acid bacteria; MOI; multiplicity of infection;
Anti-opsonic properties of staphylokinase
Keywords: Complement; Phagocytosis; Antibodies; Bacterial proteins; agr; accessory gene regulator; CHIPS; chemotaxis inhibitory protein of Staphylococcus aureus; Fc; conserved carboxy-terminal region of IgG; GFP; green fluorescent protein; IgG; immunoglobulin G; MA
HIV-1 incorporates and proteolytically processes human NDR1 and NDR2 serine-threonine kinases
Keywords: NDR; nuclear Dbf2 related; HIV-1; human immunodeficiency virus type 1; PR; protease; WT; wild type; CA; capsid; CIP; calf intestinal alkaline phosphatase; C-PKA; cAMP-dependent protein kinase; DMSO; dimethyl sulfoxide; GFP; green fluorescent protein; PMSF
Circadian rhythm of Period1 clock gene expression in NOS amacrine cells of the mouse retina
Keywords: Sensory systems; Retina and photoreceptor; Biological clock genes; Nitric oxide; GABA; Transgenic mouse; GFP; Glycine;
Development of GABAergic neurons from the ventricular zone in the superior colliculus of the mouse
Keywords: GAD67; GFP; Knock-in mouse; Cell migration; Commissural fibers;
Overexpression of squalene synthase in Eleutherococcus senticosus increases phytosterol and triterpene accumulation
Keywords: 2,4-D; 2,4-Dichlorophenoxyacetic acid; ER; endoplasmic reticulum; FPP; farnesyl diphosphate; GA3; gibberellic acid; GFP; green fluorescence protein; HPT; hygromycin phosphotransferase; MS; Murashige and Skoog; NOS; noparine synthase; RT-PCR; reverse trans
Directed evolution: selecting today's biocatalysts
Keywords: HTS; high throughput screening; epPCR; error-prone polymerase chain reaction; NRR; non-homologous random recombination; SeSaM; sequence saturation mutagenesis; ADO; assembly of designed oligonucleotides; DHR; degenerate homoduplex gene family recombinatio
Present and future of cell-penetrating peptide mediated delivery systems: “Is the Trojan horse too wild to go only to Troy?”
Keywords: CPP; cell penetrating peptide; GST; gluthation S-transferase; GFP; green fluorescent protein; FACS; fluorescence-activated cell sorter; PMO; morpholino oligomers; HPMA; N-(2-hydroxypropyl)methacrylamide; Cell delivery; Cell-penetrating peptide (CPP); Targ
Transport by vesicles of glycine- and taurine-conjugated bile salts and taurolithocholate 3-sulfate: A comparison of human BSEP with rat Bsep
Keywords: BSEP/Bsep; human/rat isoforms of the bile salt export pump; hMRP2/rMrp2; human/rat isoforms of multidrug resistance associated protein 2; hNTCP/rNtcp; human/rat isoforms of Na+-taurocholate co-transporting polypeptide; hOATP/rOatp; human/rat isoforms of o
Identification of a subtelomeric gene family expressed during the asexual-sexual stage transition in Plasmodium falciparum
Keywords: aa; amino acid; DAPI; 4,6-diamidino-2-phenylindole; FITC; fluorescein isothiocyanate; GFP; green fluorescent protein; IFA; immunofluorescent assay; PBS; phosphate-buffered saline; PCR; polymerase chain reaction; PV; parasitophorous vacuole; ORF; open read
Regulation of the cyclin D1 and cyclin A1 promoters by B-Myb is mediated by Sp1 binding sites
Keywords: GST; glutathione-S-transferase; SDS-PAGE; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; tk; thymidine kinase; GFP; green fluorescent protein; B-Myb; Sp1; Cyclin D1 promoter; Cyclin A1 promoter; Transactivation;
Utilization of mammalian cells for efficient and reliable evaluation of specificity of antibodies to unravel the cellular function of mKIAA proteins
Keywords: KIAA; KI is Kazusa DNA Research Institute and AA are reference characters; mKIAA; mouse counterparts of human KIAA; cDNA; DNA complementary to RNA; mRNA; messenger RNA; PCR; polymerase chain reaction; bp; base pair(s); kb; kilobase(s); kDa; kilodaltons; M
Regulation of exogenous gene expression in fish cells: An evaluation of different versions of the tetracycline-regulated system
Keywords: CMV; strong immediate early promoter of cytomegalovirus; DMEM; Dulbecco's modified Eagle's medium; Dox; doxycycline; EPC; carp epithelioma papulosum cell line; FBS; foetal bovine serum; GFP; green fluorescent protein; hsp70; heat-shock protein 70; LUC; lu
The mouse sphingomyelin synthase 1 (SMS1) gene is alternatively spliced to yield multiple transcripts and proteins
Keywords: bp; base pair(s); cDNA; DNA complimentary to mRNA; DAG; diacylglycerol; DMEM; Dulbecco's modified eagle's media; GFP; green fluorescent protein; GAL; galactose; GLU; glucose; HRP; horse radish peroxidase; Ig; immunoglobulin; kDa; kilodalton; oligo; oligod
Requirement of an additional Sam68 domain for inhibition of human immunodeficiency virus type 1 replication by Sam68 dominant negative mutants lacking the nuclear localization signal
Keywords: Sam68; Src-associated protein in mitosis; HIV-1; human immunodeficiency virus type 1; NLS; nuclear localization signal; CRM1; chromosome region maintenance 1; GFP; green fluorescence protein; CAT; chloramphenicol acetyltransferase; GAPDH; glyceraldyhyde-3
Estrogenic activity of alkylphenols, bisphenol S, and their chlorinated derivatives using a GFP expression system
Keywords: Alkylphenol ethoxylates; Bisphenol S; Biodegradation products; Chlorinated derivatives; Estrogenic activity; GFP;
Chemical complementation: A definitive phenotypic strategy for identifying small molecule inhibitors of elusive cellular targets
Keywords: Chemical complementation; Forward Pharmacology; Reverse Pharmacology; High-content screening; Cell-based assay; Drug discovery; Erk; extracellular signal-regulated kinase; GFP; green fluorescent protein; HTS; high-throughput screening; MAPK; mitogen-activ
VP22 enhances antibody responses from DNA vaccines but not by intercellular spread
Keywords: DNA vaccine; VP22; Intercellular spread; Protein transduction domain; GFP