کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886462 | 1537826 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transport by vesicles of glycine- and taurine-conjugated bile salts and taurolithocholate 3-sulfate: A comparison of human BSEP with rat Bsep
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کلمات کلیدی
BSEP/BsepglycoursodeoxycholateGUDCTuDCUrsodeoxycholateTaurochenodeoxycholatetcdCchenodeoxycholatecholateTaurodeoxycholatetauroursodeoxycholateTDCGCDCDJSGFPPFICHEK293UDCACDCAABC transporter - ABC حمل کنندهMOI - MELCA - ارزیابی چرخه حیاتtaurocholate - تاوروکولاتDubin-Johnson syndrome - سندروم داوین-جانسونLithocholate - لیتوچولاتBile salt - نمک صفراgreen fluorescent protein - پروتئین فلورسنت سبزmultiplicity of infection - چندین عفونتProgressive familial intrahepatic cholestasis - کلستاز داخل صفاقی خانوادگی پیشرفتهhuman embryonic kidney 293 - کلیه جنینی انسان 293glycochenodeoxycholate - گلیکوچنیدوکسوخولاتGlycocholate - گلیکوکولات
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Transport by vesicles of glycine- and taurine-conjugated bile salts and taurolithocholate 3-sulfate: A comparison of human BSEP with rat Bsep Transport by vesicles of glycine- and taurine-conjugated bile salts and taurolithocholate 3-sulfate: A comparison of human BSEP with rat Bsep](/preview/png/9886462.png)
چکیده انگلیسی
The bile salt export pump (BSEP) of hepatocyte secretes conjugated bile salts across the canalicular membrane in an ATP-dependent manner. The biliary bile salts of human differ from those of rat in containing a greater proportion of glycine conjugates and taurolithocholate 3-sulfate (TLC-S). In the present study, the transport properties of hBSEP and rBsep were investigated using membrane vesicles from HEK293 cells infected with recombinant adenoviruses containing hBSEP or rBsep cDNA. ATP-dependent uptake of radiolabeled glycine-, taurine-conjugated bile salts, and [3H]cholate was observed when hBSEP or rBsep was expressed. Comparison of initial uptake rates indicated that for both transporters, taurine-conjugated bile salts were transported more rapidly than glycine-conjugated bile salts, however, hBSEP transported glycine conjugates to an extent that was approximately 2-fold greater than rBsep. In addition, [3H]TLC-S was significantly transported by hBSEP, and hardly transported by rBsep. The mean Km value for the uptake of [3H]TLC-S by hBSEP was 9.5 ± 1.5 μM, a value similar to that for hMRP2 (8.2 ± 1.3 μM). In conclusion, both hBSEP and rBsep transport taurine-conjugated bile salts better than glycine-conjugated bile salts, but hBSEP transports glycine conjugates to a greater extent as compared to rBsep. TLC-S, which is present in human bile but not rodent bile, is more avidly transported by hBSEP compared with rBsep.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1738, Issues 1â3, 30 December 2005, Pages 54-62
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1738, Issues 1â3, 30 December 2005, Pages 54-62
نویسندگان
Hisamitsu Hayashi, Tappei Takada, Hiroshi Suzuki, Reiko Onuki, Alan F. Hofmann, Yuichi Sugiyama,