
Allosteric-site and catalytic-site ligand effects on PDE5 functions are associated with distinct changes in physical form of the enzyme
Keywords: PDE; cyclic nucleotide phosphodiesterase; IBMX; 3-isobutyl-1-methylxanthine; C domain; catalytic domain; R domain; regulatory domain; PAGE; polyacrylamide gel electrophoresis; KP; 10Â mM potassium phosphate (pH 6.8); GAF; cGMP-binding PDE, Anabaenaadenyly