کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1949950 | 1537790 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Suppression of adipogenesis program in cultured preadipocytes transfected stably with cyclooxygenase isoforms
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کلمات کلیدی
15d-PGJ2AP2ProstanoidPPARγG41815-Deoxy-Δ12,14-PGJ2LPLPVDFlipocalin-type PGD synthasePBS (−)FBSCOXIBMXphosphate-buffered saline without Ca2+ and Mg2+3-isobutyl-1-methylxanthine - 3-ایزوبوتیل-1-متیلکسانتینC/EBP - C / EBPL-PGDS - PGDSAdipocyte - آدیپوسیتcyclooxygenase - آنزیم سیکلواکسیژنازArachidonic acid - اسید آراشیدونیکsodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدOverexpression - بیش از حد بیانReverse transcriptase - ترانس کریپتاز معکوس یا وارونویسStable transfection - ترانسفکشن پایدارELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاdifferentiation medium - تمایز رسانهpolyvinylidene difluoride - دی فلوئورید پلی وینیلیدینfetal bovine serum - سرم جنین گاو3T3-L1 cell - سلول 3T3-L1Lipoprotein lipase - لیپو پروتئین لیپازmaturation medium - متوسط بالغGrowth medium - محیط رشدpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازadipocyte protein 2 - پروتئین آدیپوسیت 2CCAAT/enhancer binding protein - پروتئین اتصال CCAAT / enhancerprostaglandin - پروستاگلاندینهاGlut-4 - گلوتامین 4glucose transporter-4 - گلوکز حمل کننده-4peroxisome proliferator-activated receptor γ - گیرنده پروتئین کننده پروکسیوم فعال γ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Suppression of adipogenesis program in cultured preadipocytes transfected stably with cyclooxygenase isoforms Suppression of adipogenesis program in cultured preadipocytes transfected stably with cyclooxygenase isoforms](/preview/png/1949950.png)
چکیده انگلیسی
Prostaglandins (PGs) are known to play a variety of roles in adipocytes and precursor cells, which have the arachidonate cyclooxygenase (COX) pathway to generate several series of PGs at different stages of life cycle of adipocytes. To gain a unique insight into the specific roles of the COX isoforms during the life cycle of adipocytes, 3T3-L1 preadipocytes were stably transfected with a mammalian expression vector harboring either cDNA coding for murine COX-1 or COX-2. The cloned stable transfectants with COX-1 or COX-2 exhibited higher expression levels of their corresponding mRNA and proteins, and greater production of PGE2 upon stimulation with free arachidonic acid or A23187 than the parent cells and the transfectants with vector only. However, either type of transfectants brought about the marked reduction in the accumulation of triacylglycerols after the standard adipogenesis program. Unexpectedly, aspirin or other COX inhibitors at different phases of life cycle of adipocytes failed to reverse the reduced storage of fats. The transfectants with COX-2 were sensitive to exogenous 15-deoxy-Î12,14-PGJ2 (15d-PGJ2) and troglitazone as peroxisome proliferator-activated receptor γ (PPARγ) agonists during the maturation phase for restoring the adipogenesis. By contrast, the transfectants with COX-1 were much less sensitive, which was reflected by much lower gene expression levels of PPARγ and the related adipocyte-specific markers. Taken together, the results suggest that the sustained overexpression of either COX-1 or COX-2 resulted in the interference of adipogenesis program through a PG-independent mechanism with a different mode of action of COX isoforms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1791, Issue 4, April 2009, Pages 273-280
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1791, Issue 4, April 2009, Pages 273-280
نویسندگان
Xiaoqing Chu, Li Xu, Kohji Nishimura, Mitsuo Jisaka, Tsutomu Nagaya, Fumiaki Shono, Kazushige Yokota,