Detection and absolute quantitation of Tomato torrado virus (ToTV) by real time RT-PCR
Keywords: RT-PCR کمی; Torradovirus; Secoviridae; Detection; Diagnosis; Quantitative RT-PCR;
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Mutation of the gene encoding the ribonuclease P RNA in the hyperthermophilic archaeon Thermococcus kodakarensis causes decreased growth rate and impaired processing of tRNA precursors
Keywords: RT-PCR کمی; Hyperthermophilic archaea; Pre-tRNA processing; Ribonuclease P; Thermococcus kodakarensis; ASW; artificial sea water; CBP; calmodulin-binding peptide; 5-FOA; 5-fluoroorotic acid; PhopRNA; RNase P RNA in Pyrococcus horikoshii; qRT-PCR; quantitative RT-PCR;
Sensitive and rapid RT-qPCR quantification of pathogenic Candida species in human blood
Keywords: RT-PCR کمی; Blood; Candida; Quantitative RT-PCR;
Commitment of Annexin A2 in recruitment of microRNAs into extracellular vesicles
Keywords: RT-PCR کمی; miRNA; microRNA; EV; extracellular vesicle; qRT-PCR; quantitative RT-PCR; siRNA; small interference RNA; RNase; âribonuclease; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; ANXA1; Annexin A1; ANXA2; Annexin A2; ANXA5; Annexin A5; ANXA6; Annexin A6; E
The Trametes hirsuta 072 laccase multigene family: Genes identification and transcriptional analysis under copper ions induction
Keywords: RT-PCR کمی; White-rot fungi; Laccase multigene family; Differential expression; Phylogenetic analysis; SSH; suppression subtractive hybridization; MOS; mirror orientation selection; RACE-PCR; rapid amplification of cDNA ends; GOI; gene of interest; RQ; relative quant
scaRNAs regulate splicing and vertebrate heart development
Keywords: RT-PCR کمی; AS; alternative splicing; CHDs; congenital heart defects; RV; right ventricle; TOF; tetralogy of Fallot; scaRNAs; small cajal body-specific RNAs; CHD; congenital heart disease; SHF; second heart field; ncRNA; noncoding RNA; snRNAs; small nuclear RNAs; sno
Molecular cloning and sequence analysis of heat shock proteins 70 (HSP70) and 90 (HSP90) and their expression analysis when exposed to benzo(a)pyrene in the clam Ruditapes philippinarum
Keywords: RT-PCR کمی; cDNA; DNA complementary to RNA; HSP70; heat shock protein 70 gene; HSP90; heat shock protein 90 gene; XMEs; xenobiotic metabolizing enzymes; BaP; benzo(a)pyrene; PCR; polymerase chain reaction; RT-PCR; reverse transcriptase PCR; qRT-PCR; quantitative RT-P
C/EBPβ is a transcriptional key regulator of IL-36α in murine macrophages
Keywords: RT-PCR کمی; BMDMs; bone marrow-derived macrophages; bZIP; basic leucine zipper; ChIP; chromatin immunoprecipitation; half-CRE; half-cAMP response element; IL-1Ra; IL-1R antagonist; IL-36Ra; IL-36R antagonist; IL-1RAcP; IL-1R accessory protein; qPCR; quantitative PCR;
Follistatin-like 1 attenuates differentiation and survival of erythroid cells through Smad2/3 signaling
Keywords: RT-PCR کمی; Erythropoiesis; Erythroid cells; Differentiation; Cell survival; FSTL1; Smad2/3; BMP; bone morphogenetic protein; DMEM; Dulbecco's modified Eagle's medium; PCR; polymerase chain reaction; PE; phenylephrine; qRT-PCR; quantitative RT-PCR; TGF; transforming
Macrophages and galectin 3 play critical roles in CVB3-induced murine acute myocarditis and chronic fibrosis
Keywords: RT-PCR کمی; VM; viral myocarditis; CVB3; coxsackievirus B3; DCM; dilated cardiomyopathy; HF; heart failure; MOI; multiplicity of infection; LipClod; liposome encapsulated clodronate; Gal-3; galectin 3; dpi; days post-infection; Lgals3â/â; Gal-3-deficient mice; WT
Isolation and expression analysis of 18 CsbZIP genes implicated in abiotic stress responses in the tea plant (Camellia sinensis)
Keywords: RT-PCR کمی; Tea plant (Camellia sinensis); Basic leucine zipper protein (bZIP); Genes expression; Abiotic stress; ABA; abscisic acid; ABF; ABA responsive element binding factor; ABRE; ABA responsive elements; bZIP; basic leucine zipper protein; CBF; C-repeat/dehydrat
Piwi-like 1 and 4 gene transcript levels are associated with clinicopathological parameters in renal cell carcinomas
Keywords: RT-PCR کمی; Piwil; Piwi-like; ccRCC; Clear cell renal cell carcinoma; Piwi-like genes; Clear cell renal cell carcinoma; Quantitative RT-PCR; Age; Normal renal tissue; Tumorigenesis;
Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells
Keywords: RT-PCR کمی; hAD-MSCs; human adipose tissue-derived mesenchymal stem cells; TiO2; titanium dioxide; SIM; simvastatin; OPN; osteopontin protein; SPP1; osteopontin mRNA; OPG; osteoprotegerin protein; TNFRSF11B; osteoprotegerin mRNA; OC; osteocalcin protein; BGLAP; osteo
Dupilumab improves the molecular signature in skin of patients with moderate-to-severe atopic dermatitis
Keywords: RT-PCR کمی; Atopic dermatitis; dupilumab; IL-4 receptor α inhibition; transcriptome; gene expression; skin; TH2 axis; AD; Atopic dermatitis; CsA; Cyclosporin A; DC; Dendritic cell; EASI; Eczema Area and Severity Index; IL-4Rα; IL-4 receptor α; LS; Lesional skin bi
25(OH) vitamin D suppresses macrophage adhesion and migration by downregulation of ER stress and scavenger receptor A1 in type 2 diabetes
Keywords: RT-PCR کمی; CVD; cardiovascular disease; T2DM; type 2 diabetes mellitus; 25(OH)D; 25-hydroxy vitamin D; MCP-1; monocyte chemotactic protein 1; CCR2; chemokine (C-C motif) receptor 2; ER; endoplasmic reticulum; SR-A1; scavenger receptor class A type 1; M-CSF; macropha
Differential microRNA expression between shoots and rhizomes in Oryza longistaminata using high-throughput RNA sequencing
Keywords: RT-PCR کمی; Oryza longistaminata; Deep sequencing; miRNA; Quantitative RT-PCR
Molecular characterization and mRNA expression of ribosomal protein L8 in Rana nigromaculata during development and under exposure to hormones
Keywords: RT-PCR کمی; Ribosomal protein L8; Rana nigromaculata; Endocrine disrupting chemical; Quantitative RT-PCR; Reference gene
VDR primary targets by genome-wide transcriptional profiling
Keywords: RT-PCR کمی; 1α,25(OH)2D3; 1α,25-dihydroxyvitamin D3; ChIP; chromatin immunoprecipitation; ChIP-Seq; ChIP-sequencing; DAVID; Database for Annotation, Visualization and Integrated Discovery; FVKM; fragments per virtual kb per million; log2fc; log2 fold change; H3K4me
Deletion of olfactomedin 2 induces changes in the AMPA receptor complex and impairs visual, olfactory, and motor functions in mice
Keywords: RT-PCR کمی; EMT; elevated plus maze test; ERG; electroretinogram; KO; knockout; Olfm2; olfactomedin 2; Olfm2-KO; Olfm2 knockout; β-gal; β-galactosidase; PFA; paraformaldehyde; Q-PCR; quantitative RT-PCR; RGC; retinal ganglion cell; VEP; visual evoked potential; WT;
Cloning and characterization of neoplasia-related genes in flat oyster Ostrea edulis
Keywords: RT-PCR کمی; C1qTNF; complement 1q-tumour necrosis factor; DN; disseminated neoplasia; NDPk; nucleoside diphosphate kinase; ORF; open reading frame; p23; progesterone E synthase 3; Q-PCR; quantitative RT-PCR; RACK; receptor for activated kinase PKC; RPA3; replication
Effects of rhinovirus species on viral replication and cytokine production
Keywords: RT-PCR کمی; Asthma; cellular cytotoxicity; chemokine; cytokine; rhinovirus; ALI; Air-liquid interface; HSEC; Human sinus epithelial cell; LDH; Lactate dehydrogenase; PBEC; Primary bronchial epithelial cell; PI; Postinfection; qRT-PCR; Quantitative RT-PCR; RV; Rhinovi
1,25(OH)2 vitamin D suppresses macrophage migration and reverses atherogenic cholesterol metabolism in type 2 diabetic patients
Keywords: RT-PCR کمی; MCP-1; monocyte chemotactic protein 1; ER; endoplasmic reticulum; CVD; cardiovascular disease; 1,25(OH)2D; 1,25-dihydroxy vitamin D; CCR2; chemokine (C-C motif) receptor 2; IFN; interferon; IL; interleukin; IC; immunocomplex plus lipopolysaccharide; 25(
Hd86 mRNA expression profile in Hyalomma scupense life stages, could it contribute to explain anti-tick vaccine effect discrepancy between adult and immature instars?
Keywords: RT-PCR کمی; Hyalomma scupense tick species; Hd86 transcript; Quantitative RT-PCR; Expression profile; Hd86 vaccine; Immature and adult stages;
Lipopolysaccharide-induced TNF-α factor in grass carp (Ctenopharyngodon idella): Evidence for its involvement in antiviral innate immunity
Keywords: RT-PCR کمی; LITAF; TNF-α; Grass carp (Ctenopharyngodon idella); Grass carp reovirus; Quantitative RT-PCR;
Deletion in the N-terminal half of olfactomedin 1 modifies its interaction with synaptic proteins and causes brain dystrophy and abnormal behavior in mice
Keywords: RT-PCR کمی; MRI; Magnetic resonance imaging; Olfm1; Olfactomedin 1; Olfm1-KO; Olfm1 knockout; Q-PCR; Quantitative RT-PCR; Olfactomedin 1; AMPA receptor; Neurobiology; Proteomics; Cell signaling; Mouse; Anxiety behavior; Olfactory defects;
S[+] Apomorphine is a CNS penetrating activator of the Nrf2-ARE pathway with activity in mouse and patient fibroblast models of amyotrophic lateral sclerosis
Keywords: RT-PCR کمی; ALS; amyotrophic lateral sclerosis; ARE; antioxidant response element; carboxy-H2DCFDA; 6-carboxy-2â²,7â²-dichlorodihydrofluorescein diacetate; CNS; central nervous system; DCF; dichlorofluorescein; MEFs; mouse embryonic fibroblasts; Nrf2; nuclear eryth
Estimation of paracrine signaling using quantitative RT-PCR from multiple patchy lesion samples
Keywords: RT-PCR کمی; Paracrine; Cytokine; Inflammation; Organ injury; Quantitative RT-PCR; Correlation analysisEAM, experimental autoimmune myocarditis; NCNI, non-cardiomyocyte non-inflammatory; Lcn2/NGAL, lipocalin-2/neutrophil gelatinase-B associated lipocalin; Cox2, cycloo
Increased expression of USP22 is associated with disease progression and patient prognosis of salivary duct carcinoma
Keywords: RT-PCR کمی; Salivary duct carcinoma; USP22; Prognosis; Immunohistochemistry; Quantitative RT-PCR
Nonsense-mediated mRNA decay was demonstrated in two hypofibrinogenemias caused by heterozygous nonsense mutations of FGG, Shizuoka III and Kanazawa II
Keywords: RT-PCR کمی; hypofibrinogenemia; nonsense-mediated mRNA decay (NMD); quantitative RT-PCR
Promoter regions of potato vacuolar invertase gene in response to sugars and hormones
Keywords: RT-PCR کمی; Cis-acting element; Potato; StvacINV1 promoter; Transcriptional regulation; ABA; abscisic acid; BAP; 6-benzylaminopurine; GA; gibberellic acid; GARE; GA-response element; GUS; β-glucuronidase; hiTAIL-PCR; high-efficient thermal asymmetric interlaced poly
Genetic and phenotypic characterization of the RyhB regulon in Salmonella Typhimurium
Keywords: RT-PCR کمی; RyhB; Iron homeostasis; Salmonella Typhimurium; Quantitative RT-PCR; Phenotypic assays
Identification of cell-specific patterns of reference gene stability in quantitative reverse-transcriptase polymerase chain reaction studies of embryonic, placental and neural stem models of prenatal ethanol exposure
Keywords: RT-PCR کمی; Reference gene; Normalization; Alcohol; Quantitative RT-PCR; Real-time PCR; Stem cells
Identification of ORF sequences and exercise-induced expression change in thoroughbred horse OXCT1 gene
Keywords: RT-PCR کمی; OXCT1; 3-oxoacid CoA transferase 1; cDNA; complementary DNA; TCA; tricarboxylic acid; ATP; adenosine triphosphate; GTP; guanosine triphosphate; ORF; open reading frame; OXCT1 gene; Quantitative RT-PCR; Thoroughbred horse; Exercise;
Influence of BONITmatrix® and OSSA NOVA on the expression of bone specific genes
Keywords: RT-PCR کمی; Bone defect; Quantitative RT-PCR; Osteoblast; Osteoclast; Marker genes;
Changes in condylar cartilage after anterior mandibular displacement in juvenile pigs
Keywords: RT-PCR کمی; Collagen; Matrix metalloproteinase; Quantitative RT-PCR; Temporomandibular joint; Vascular endothelial growth factor;
Bone substitution materials on the basis of BONITmatrix® up-regulate mRNA expression of IGF1 and Col1a1
Keywords: RT-PCR کمی; Bone defect; Quantitative RT-PCR; Collagen; IGF; Growth factors;
Modification of N-glycosylation modulates the secretion and lipolytic function of apoptosis inhibitor of macrophage (AIM)
Keywords: RT-PCR کمی; AIM; apoptosis inhibitor of macrophage; SRCR; scavenger receptor cysteine-rich; FSP27; fat-specific protein 27; TLR; toll-like receptor; FAS; fatty acid synthase; QPCR; quantitative RT-PCR; IL-6; interleukin-6; Saa-3; serum amyloid A-3; DEX; dexamethasone
Opioid receptor expression in human brain and peripheral tissues using absolute quantitative real-time RT-PCR
Keywords: RT-PCR کمی; Opioid receptors; Quantitative RT-PCR; Brain; Peripheral tissues;
Selection of suitable inner reference genes for relative quantification expression of microRNA in wheat
Keywords: RT-PCR کمی; Non-coding RNA; Internal controls gene; Quantitative RT-PCR; Puccinia striiformis f. sp. Tritici; Wheat
Different associations of estrogen receptor β isoforms, ERβ1 and ERβ2, expression levels with tumor size and survival in early- and late-onset breast cancer
Keywords: RT-PCR کمی; Estrogen receptor beta; Estrogen receptor alpha; Clinical outcome; Early- and late-onset breast cancers; Quantitative RT-PCR;
Histologic subtypes, immunohistochemistry, FISH or molecular screening for the accurate diagnosis of ALK-rearrangement in lung cancer: A comprehensive study of Caucasian non-smokers
Keywords: RT-PCR کمی; Non-small cell lung carcinoma; EML4-ALK fusion protein; Reverse transcriptase polymerase chain reaction; Quantitative RT-PCR; Fluorescent in situ hybridization; Immunohistochemistry; Pathology;
Comparative profiling of miRNA expression of lung adenocarcinoma cells in two-dimensional and three-dimensional cultures
Keywords: RT-PCR کمی; ECM; extracellular matrix; BM; basement membrane matrix; rBM 3-D; reconstituted basement membrane matrix based three-dimensional organotypic culture; miRNA; microRNA; F-actin; filamentous actin; qRT-PCR; quantitative RT-PCR; pri-miR-21; primary miR-21; VM
GacA directly regulates expression of several virulence genes in Pseudomonas syringae pv. tabaci 11528
Keywords: RT-PCR کمی; QS; quorum sensing; EMSA; electrophoretic mobility shift assay; Fur; ferric uptake regulator; N-Ahls; N-acyl homoserine lactone; Ct; cycle threshold; TTSS; type III secretion system; GacA; Iron availability; Quantitative RT-PCR; Electrophoretic mobility s
Identification, basic characterization and evolutionary analysis of differentially spliced mRNA isoforms of human YAP1 gene
Keywords: RT-PCR کمی; bp; base pair; cDNA; DNA complementary to RNA; PCR; polymerase chain reaction; WW domain; tryptophan-tryptophan domain; Alternative splicing; WW domains; Leucine zipper; Quantitative RT-PCR;
Telomere dysfunction induced by chemotherapeutic agents and radiation in normal human cells
Keywords: RT-PCR کمی; β2-M; β2-microglubin; ConA; concanavalin A; DOX; doxorubicin; DSB; double strand DNA break; hTERC; human telomerase RNA component; hTERT; human telomerase reverse transcriptase; IF; immunofluorescence; qRT-PCR; quantitative RT-PCR; TIF; telomere dysfunc
Long-lasting transcriptional refractoriness triggered by a single exposure to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyrimidine
Keywords: RT-PCR کمی; Cdkn1a; cyclin-dependent kinase inhibitor 1a; CDS; coding sequence; DA; dopamine; DOPAC; 3-4-dihydroxphenylacetic acid; EDTA; ethylenediaminetetraacetic acid; Edg3; endothelial differentiation, sphingolipid G-protein-coupled receptor 3; Fosb; FBJ murine o
Detection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan®MGB probes
Keywords: RT-PCR کمی; TSWV; Tospovirus; Diagnosis; Quantitative RT-PCR
The plant limonoid 7-oxo-deacetoxygedunin inhibits RANKL-induced osteoclastogenesis by suppressing activation of the NF-κB and MAPK pathways
Keywords: RT-PCR کمی; RANKL; receptor activator of NF-κB ligand; 7-OG; 7-oxo-7-deacetoxygedunin; TRAP; tartrate resistance acid phosphatase; M-CSF; monocyte colony stimulating factor; qRT-PCR; quantitative RT-PCR; Plant limonoid; Osteoclast; RANKL; NF-κB pathway; MAPK pathwa
Expression profiles of extracellular superoxide dismutase during mouse organogenesis
Keywords: RT-PCR کمی; EC-SOD; Mouse; Embryo; Extraembryonic tissue; Antioxidant enzyme; Organogenesis; Quantitative RT-PCR; Western blotting; In situ hybridization
Protocol for the assessment of viral retention capability of membranes
Keywords: RT-PCR کمی; MS2 phage; Ultrafiltration; Microfiltration; Virus; PFU; Quantitative RT-PCR;