
Construction of a fully active Cys-less elongation factor Tu: Functional role of conserved cysteine 81
Keywords: aa-tRNA; aminoacyl-tRNA; EF-Tu; elongation factor Tu; FRET; fluorescence resonance energy transfer; sm; single molecule; LB; Luria-Bertani; TPCK; N-tosyl-l-phenylalanylchloromethane; Pi; inorganic phosphate; IPTG; isopropyl-β-d-thiogalactopyranoside; S