کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10106915 | 1613745 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Potential role of myo-inositol to improve ischemic stroke outcome in diabetic mouse
ترجمه فارسی عنوان
نقش بالقوه میوآنزیتول برای بهبود سکته مغزی ایسکمیک در موش دیابتی
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کلمات کلیدی
BCANVUEBSSAQP4STZTMCAOMCAOTransient MCAODCIOGDPFAHBSSt-PATTCCCAd-chiro-inositolDPBSFBSICAECADMEMPVDFGFAPMCATBS2,3,5-triphenyltetrazolium chloride - 2،3،5-trihenyltetrazolium chloride3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-diphenyltetrazolium bromideDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoMTT - MTTBrain edema - ادم مغزیstreptozotocin - استرپتوزوتوسینmiddle cerebral artery occlusion - انسداد شریان (سرخرگ) مغزی میانیDiabetes - بیماری قندANOVA - تحلیل واریانس Analysis of varianceone-way analysis of variance - تحلیل واریانس یک راههTris-buffered saline - تریس بافر شورTransporters - حمل و نقلCNS - دستگاه عصبی مرکزیEarle’s Balanced Salt Solution - راه حل نمکی متعادل ارلBBB - سد خونی مغزیfetal bovine serum - سرم جنین گاوIschemic stroke - سکته مغزی ایسکمیکStroke Therapy Academic Industry Roundtable - سکته مغزی جلسه علمی صنعت گردشگریcentral nervous system - سیستم عصبی مرکزیmiddle cerebral artery - شریان مغزی میانیexternal carotid artery - شریان کاروتید خارجیinternal carotid artery - شریان کاروتید داخلیcommon carotid artery - شریان کاروتید مشترکPolyvinylidene difluoride membrane - غشاء دیفلوئید پلی وینیلیدینTissue-plasminogen activator - فعال کننده بافت-پلاسمینژنBlood-brain barrier - مانع خون مغزیHank’s balanced salt solution - محلول نمک متعادل هانکMyo-inositol - میو-آنزیتولneurovascular unit - واحد عصبی عضلانیparaformaldehyde - پارافرمالدهیدGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالStair - پله
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
چکیده انگلیسی
Brain edema is one of the critical factors causing hightened disability and mortality in stroke patients, which is exaggerated further in diabetic patients. Organic osmolytes could play a critical role in the maintenance of cytotoxic edema. The present study was aimed to assess the role of myo-inositol, an organic osmolyte, on stroke outcome in diabetic and non-diabetic animals. In situ brain perfusion and acute brain slice methods were used to assess transport of myo-inositol across the blood-brain barrier and uptake by brain cells using non-diabetic (C57BL/6) and diabetic (streptozotocin-induced) mice, respectively. In vitro studies were conducted to assess the role of myo-inositol during and after ischemia utilizing oxygen glucose deprivation (OGD) and reperfusion. Further, the expression of transporters, such as SGLT6, SMIT1 and AQP4 were measured using immunofluorescence. Therapeutic efficacy of myo-inositol was evaluated in a transient middle cerebral artery occlusion (tMCAO) mouse model using non-diabetic (C57BL/6) and diabetic (db/db) mice. Myo-inositol release from and uptake in astrocytes and altered expression of myo-inositol transporters at different OGD timepoints revealed the role of myo-inositol and myo-inositol transporters during ischemia reperfusion. Further, hyperglycemic conditions reduced myo-inositol uptake in astrocytes. Interestingly, in in-vivo tMCAO, infarct and edema ratios following 24â¯h reperfusion decreased in myo-inositol treated mice. These results were supported by improvement in behavioral outcomes in open-field test, corner test and neurological score in both non-diabetic and db/db animals. Our data suggest that myo-inositol and myo-inositol transporters may provide neuroprotection during/following stroke both in non-diabetic and diabetic conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1699, 15 November 2018, Pages 166-176
Journal: Brain Research - Volume 1699, 15 November 2018, Pages 166-176
نویسندگان
Heidi Villalba, Kaushik Shah, Thamer H. Albekairi, Ali E. Sifat, Bhuvaneshwar Vaidya, Thomas J. Abbruscato,