کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10137385 | 1645756 | 2018 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of classical swine fever virus (CSFV) E2 and Erns antibody (IgG, IgA) in oral fluid specimens from inoculated (ALD strain) or vaccinated (LOM strain) pigs
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
The objective of this study was to describe oral fluid and serum antibody (IgG, IgA) responses against classical swine fever virus (CSFV) E2 and Erns proteins in pigs (nâ=â60) inoculated with a moderately virulent field strain (ALD, nâ=â30) or a modified live virus vaccine strain (LOM, nâ=â30). Oral fluid (nâ=â1391) and serum (nâ=â591) samples were collected from individually-housed pigs between day post inoculation (DPI) â14 to 28. Testing revealed the synchronous appearance of E2- and Erns-specific IgG and IgA antibodies in serum and oral fluids over time, with E2 and Erns IgG ELISAs providing better diagnostic performance than the IgA ELISAs. Overall the data suggest the feasibility of large-scale, cost-effective screening of populations for CSFV using oral fluid samples. Given the historic issues of cross-reactivity among pestiviruses, future research should focus on the development of CSFV-specific testing platforms for the detection of E2 and/or Erns IgG in oral fluid, ideally to be used in combination with DIVA vaccines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 224, October 2018, Pages 70-77
Journal: Veterinary Microbiology - Volume 224, October 2018, Pages 70-77
نویسندگان
Yaowalak Panyasing, Roongroje Thanawongnuwech, Ju Ji, Luis Giménez-Lirola, Jeffrey Zimmerman,