Evaluation of salivary DNA obtained from dental prosthesis and its applicability in forensic investigations

• Salivary DNA extracted after 1 week and 1 month from the dental prosthesis.
• 15 STRs were amplified via polymerase chain reaction.
• Samples showed sufficient amount of DNA for investigations even after storage period.

AimThis study evaluated the salivary DNA obtained from dental prosthesis after a period of storage and its applicability in human identification.Material and methodsIn first phase, DNA was extracted after a span of 1 week and 1 month from the dental prosthesis dipped in whole saliva for 15 min. It was followed by PCR and electrophoresis.In second phase, from extracted DNA samples 15 STRs (short tandem repeats) of human genomic DNA were amplified via polymerase chain reaction.Results & conclusionDNA isolated from saliva stained dental prosthesis after a period of storage and the techniques employed are adequate for further forensic analysis.