کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10162645 1114336 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation of Calcium Phosphate Nanocapsule Including Deoxyribonucleic Acid-Polyethyleneimine-Hyaluronic Acid Ternary Complex for Durable Gene Delivery
ترجمه فارسی عنوان
آماده سازی نانوکاپسول فسفات کلسیم شامل مجتمع ترانسوریک اسید دز اری ریبونوکلئیک اسید پلی اتیلنیمین-هیالورونیک برای تحویل ژنی پایدار
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
چکیده انگلیسی
Our plasmid delivery systems comprising deoxyribonucleic acid (DNA), polyethyleneimine (PEI), and hyaluronic acid (HA) have already achieved the high‐extragene expression in tumor tissues. Repeated transfection with certain cytokine genes effectively induced tumor regression and complete disappearance of the tumor in some cases. Frequent injection is sometimes difficult depending on the tumor site. However, single injection often leads to an unsatisfactory efficacy owing to the short duration of the gene expression. In this study, we prepared calcium phosphate (CaP) nanocapsule including plasmid DNA complexes as a durable gene transfection system, which would be slowly degraded, and release DNA complex in the body. CaP nanocupsule including DNA complexes with a diameter of approximately 200 nm was prepared by immersing HA‐coated DNA-PEI complex in simulated body fluid. It showed gene expression in cultured cells with duration longer than 2 weeks. By this slow‐releasing system, significant tumor‐growth suppression and, finally, complete tumor disappearance were observed after single injection into the tumor. Capsulated DNA complex with Ca thus seems promising as a sustained gene expression device. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:179-184, 2014
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 103, Issue 1, January 2014, Pages 179-184
نویسندگان
, , ,