کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10162719 | 1114349 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Contribution of Radixin to P-Glycoprotein Expression and Transport Activity in Mouse Small Intestine In Vivo
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Contribution of Radixin to P-Glycoprotein Expression and Transport Activity in Mouse Small Intestine In Vivo Contribution of Radixin to P-Glycoprotein Expression and Transport Activity in Mouse Small Intestine In Vivo](/preview/png/10162719.png)
چکیده انگلیسی
The ERM proteins, ezrin, radixin, and moesin, are membrane-cytoskeleton crosslinkers with multiple physiological functions. We previously showed that radixin is involved in posttranslational regulation of P-glycoprotein (P-gp) in human hepatoblastoma HepG2 cells. Here, we investigated the physiological role of radixin in regulating P-gp expression and activity in the small intestine by comparing wild-type- and radixin knockout (Rdx) mice. In intestinal tissue homogenates, P-gp protein levels increased markedly from the upper part to the lower part of the small intestine in both wild-type- and Rdxâ/â mice. In the membrane fractions, a similar pattern was seen in wild-type mice. However, the membrane expression of P-gp protein remained at the same level from the upper to the lower part of the small intestine in Rdxâ/â mice. When rhodamine123 (Rho123), a substrate of P-gp, was orally administered to Rdxâ/â and wild-type mice, the absorption phase of Rho123 was greater in Rdxâ/â than in wild-type mice, whereas the elimination phase in Rdxâ/â mice was not different from that of wild-type mice. Our results indicate that radixin plays an important role in regulating P-gp localization and P-gp functional activity at the intestinal membrane. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:2875-2881, 2013
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 102, Issue 8, August 2013, Pages 2875-2881
Journal: Journal of Pharmaceutical Sciences - Volume 102, Issue 8, August 2013, Pages 2875-2881
نویسندگان
Kentaro Yano, Takumi Tomono, Riyo Sakai, Takashi Kano, Kaori Morimoto, Yukio Kato, Takuo Ogihara,