کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10233091 | 42678 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dynamic flux balance analysis for pharmaceutical protein production by Pichia pastoris: Human growth hormone
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The influence of methanol feeding rate on intracellular reaction network of recombinant human growth hormone (rhGH) producing Pichia pastoris was investigated at three different specific growth rates, namely, 0.02 (MS-0.02), 0.03 (MS-0.03), and 0.04 hâ1 (MS-0.04) where Period-I (33 â¤Â t < 42 h) includes the early exponential growth phase; Period-II (42 â¤Â t < 48 h) is the exponential growth phase where the specific cell growth rate decreases; Period-III (48 â¤Â t â¤Â 51 h) is the exponential growth phase where rhGH concentration was the highest; and Period-IV (t > 51 h) is the diminution phase for rhGH and cell synthesis. In Period-I, almost all of the formaldehyde entered the assimilatory pathway, at MS-0.02 and MS-0.03, whereas, at MS-0.04 high methanol feeding rate resulted in an adaptation problem. In Period-III, only at MS-0.02 co-carbon source sorbitol uptake-flux was active showing that sorbitol uptake does not affected from the predetermined feeding rate of methanol at μ0 > 0.02 hâ1. The biomass synthesis flux value was the highest in Period-I, -II and -III, respectively at MS-0.03 & MS-0.04, MS-0.04 and MS-0.02; whereas, rhGH flux was the highest in Period-I, -II, and -III, respectively at MS-0.03, MS-0.02 and MS-0.03. Based on the fluxes, Period-I should start with MS-0.03 methanol feeding rate and starting from the middle of Period-II methanol feeding rate should be shifted to MS-0.02.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 48, Issue 3, 7 March 2011, Pages 209-216
Journal: Enzyme and Microbial Technology - Volume 48, Issue 3, 7 March 2011, Pages 209-216
نویسندگان
Pınar Ãalık, Merve Åahin, Hatice TaÅpınar, Elif Å. Soyaslan, Bahar Ä°nankur,