کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10276213 | 463421 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Study of the DNA hybridization transduction behavior of a quinone-containing electroactive polymer by cyclic voltammetry and electrochemical impedance spectroscopy
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We report the characterization of a new bifunctional electroactive polymer, poly(5-hydroxy-1,4-naphthoquinone (juglone)-co-5-hydroxy-3-thioacetic acid-1,4-naphthoquinone), which can be used for direct electrochemical detection of DNA hybridization. The polymer structure was characterized based on molecular orbital calculations and ex situ and in situ FT-IR spectroscopy. The quinone group integrated in the polymer presents very stable electroactivity in neutral aqueous medium and can act as a redox marker for hybridization. The carboxylic function allows the preparation of oligonucleotides (ODN) probe-modified films by covalent binding. The mechanism of hybridization detection was studied by cyclic voltammetry and electrochemical impedance spectroscopy. The “signal-on” upon hybridization could be attributed to a change in conformation from random ODN coils on the probe-modified film to helix double stranded ODNs upon hybridization.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 577, Issue 1, 15 March 2005, Pages 155-165
Journal: Journal of Electroanalytical Chemistry - Volume 577, Issue 1, 15 March 2005, Pages 155-165
نویسندگان
B. Piro, J. Haccoun, M.C. Pham, L.D. Tran, A.Rubin A.Rubin, H. Perrot, C. Gabrielli,