New enzymatic time-temperature integrator (TTI) that uses laccase

► A new enzymatic TTI prototype was developed using laccase. ► The laccase TTI regularly changed in color according to zero-order reaction kinetics. ► The activation energy (Ea) of the laccase TTI ranged from 43.9 to 46.9 kJ/mol. ► The Ea can cover food quality changes such as enzymatic changes, hydrolysis, and lipid oxidation.