کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10395396 888977 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cellulosic ethanol production by Zymomonasmobilis harboring an endoglucanase gene from Enterobactercloacae
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Cellulosic ethanol production by Zymomonasmobilis harboring an endoglucanase gene from Enterobactercloacae
چکیده انگلیسی
Enterobactercloacae was isolated from the gut of the wood feeding termite, Heterotermesindicola, and a 2.25-kb fragment conferring cellulase activity was cloned in Escherichiacoli. The cloned fragment contained a 1083-bp ORF which could encode a protein belonging to glycosyl hydrolase family 8. The cellulase gene was introduced into Zymomonasmobilis strain Microbial Type Culture Collection centre (MTCC) on a plasmid and 0.134 filter paper activity unit (FPU)/ml units of cellulase activity was observed with the recombinant bacterium. Using carboxymethyl cellulose and 4% NaOH pretreated bagasse as substrates, the recombinant strain produced 5.5% and 4% (V/V) ethanol respectively, which was threefold higher than the amount obtained with the original E.cloacae isolate. The recombinant Z. mobilis strain could be improved further by simultaneous expression of cellulase cocktails before utilizing it for industrial level ethanol production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 102, Issue 3, February 2011, Pages 2585-2589
نویسندگان
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