کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10395396 | 888977 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cellulosic ethanol production by Zymomonasmobilis harboring an endoglucanase gene from Enterobactercloacae
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cellulosic ethanol production by Zymomonasmobilis harboring an endoglucanase gene from Enterobactercloacae Cellulosic ethanol production by Zymomonasmobilis harboring an endoglucanase gene from Enterobactercloacae](/preview/png/10395396.png)
چکیده انگلیسی
Enterobactercloacae was isolated from the gut of the wood feeding termite, Heterotermesindicola, and a 2.25-kb fragment conferring cellulase activity was cloned in Escherichiacoli. The cloned fragment contained a 1083-bp ORF which could encode a protein belonging to glycosyl hydrolase family 8. The cellulase gene was introduced into Zymomonasmobilis strain Microbial Type Culture Collection centre (MTCC) on a plasmid and 0.134Â filter paper activity unit (FPU)/ml units of cellulase activity was observed with the recombinant bacterium. Using carboxymethyl cellulose and 4% NaOH pretreated bagasse as substrates, the recombinant strain produced 5.5% and 4% (V/V) ethanol respectively, which was threefold higher than the amount obtained with the original E.cloacae isolate. The recombinant Z. mobilis strain could be improved further by simultaneous expression of cellulase cocktails before utilizing it for industrial level ethanol production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 102, Issue 3, February 2011, Pages 2585-2589
Journal: Bioresource Technology - Volume 102, Issue 3, February 2011, Pages 2585-2589
نویسندگان
P. Thirumalai Vasan, P. Sobana Piriya, D. Immanual Gilwax Prabhu, S. John Vennison,