کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429474 | 909794 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Real-time detection of β1 integrin expression on MG-63 cells using electrochemical impedance spectroscopy
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Beta 1 integrin is a membrane protein responsible for attachment and migration of osteosarcoma cells. In this study, expression of β1 integrin on MG-63 cells, a human osteogenic sarcoma cell line, was monitored using electrochemical impedance spectroscopy (EIS). ITO-based biochips were developed using a semiconductor technique. Differences in electric resistance (ÎR) were measured continuously when cells binding with anti-β1 integrin antibody coagulated with nano-scale gold particles. The results of the EIS system were compared with traditional immunofluorescence staining. We found that sample chambers with higher cell densities had larger ÎR values. When the cell densities increased from 5 Ã 104 cells/ml to 5 Ã 105 cells/ml, the ÎR value dose-dependently increased from 14 Ω to 37 Ω. In addition, a highly linear relationship (correlation coefficient, 0.921) was found between the ÎR values and the corresponding fluorescence intensities (p < 0.05). These results suggest that electrochemical impedance spectroscopy can be a useful tool for evaluating β1 integrin expression on cell membranes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 28, Issue 1, 15 October 2011, Pages 221-226
Journal: Biosensors and Bioelectronics - Volume 28, Issue 1, 15 October 2011, Pages 221-226
نویسندگان
Chun-Yen Lin, Nai-Chia Teng, Sung-Chih Hsieh, Yung-Sheng Lin, Wei-Jen Chang, Sheng-Yi Hsiao, Hui-Shun Huang, Haw-Ming Huang,