Enantioseparation of dansyl amino acids by a novel permanently positively charged single-isomer cyclodextrin: Mono-6-N-allylammonium-6-deoxy-β-cyclodextrin chloride by capillary electrophoresis

A permanently positively charged single-isomer β-CD, mono-6-N-allylammonium-6-deoxy-β-CD chloride, ALAM-β-CD, has been synthesized and successfully used as chiral selector in capillary electrophoresis for enantioseparation of amino acids and dansyl amino acids. The effects of pH, CD's concentration and capillary length on enantioseparation were studied in order to investigate its potential as chiral selector for acidic racemates. By increasing buffer's pH and CD's concentration, fairly robust separations were achieved. Good separations were also obtained with a shorter capillary. In addition, baseline enantiomeric separations of a mixture of six pairs of dansyl dl-amino acids were achieved under different pH conditions.