Barley γ3-hordein: Glycosylation at an atypical site, disulfide bridge analysis, and reactivity with IgE from patients allergic to wheat

► The γ3 hordein, a barley prolamin, is glycosylated at an atypical Asn-Leu-Cys site. ► Glycosylated hordein molecules are targeted through the Golgi apparatus. ► Native γ3 hordein disulfide bridge folding is similar to that of wheat γ46-gliadin. ► γ3 hordein binds IgE from wheat-allergic patients mainly after reduction-alkylation. ► γ3 hordein glycosylation is not involved in IgE binding and even decreases it.