کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10537953 | 962885 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteome analysis of rat pancreas induced by pancreatectomy
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
RPEPBSFBSSTZMALDI-TOFFPLC2-DEMS/MS - MS / MSMTT - MTTstreptozotocin - استرپتوزوتوسین2-dimensional electrophoresis - الکتروفورز 2 بعدیRegeneration - باززاییfetal bovine serum - سرم جنین گاوfast protein liquid chromatography - سریع کروماتوگرافی مایع پروتئینMass spectrometry - طیف سنجی جرمیTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیPhosphate buffered saline - فسفات بافر شورpancreatectomy - پانکراتکتومی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Proteome analysis of rat pancreas induced by pancreatectomy Proteome analysis of rat pancreas induced by pancreatectomy](/preview/png/10537953.png)
چکیده انگلیسی
The previous study demonstrated that the streptozotocin (STZ)-induced diabetic mice can be cured by injecting the regenerating pancreatic extract (RPE) of the partially pancreatectomized Wistar-Kyoto rats. In this study, to characterize the complex pattern of protein expression in RPE, the proteins of altered expression level after the pancreatectomy were identified by 2-dimensional electrophoresis (2-DE) and mass spectrometry. Of 76 significantly up- or down-regulated protein spots, 61 were identified by MALDI-TOF/MS. Moreover, the whole RPE was fractionated into 4 groups using an anion-exchange chromatography and each fraction's cell proliferating activity was measured by MTT assay. Compared to the normal pancreatic extract, fraction 3 and 4 of RPE showed the maximal cell proliferating activity. On 2-DE of 3 and 4 fractions, a total of 10 spots, which are differentially expressed after the pancreatectomy, were identified by MS/MS. Of these identified proteins, Reg III which might be functionally associated with well known regenerating factor (Reg I) was found. Taken together, our results demonstrated that the differential protein expression associated with pancreas regeneration could be sought by 2-DE and mass spectroscopy and suggested that the pre-fractionation method combined with in vitro cell proliferation assay is effectively used to pinpoint the active components for pancreas regeneration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1749, Issue 1, 20 May 2005, Pages 23-32
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1749, Issue 1, 20 May 2005, Pages 23-32
نویسندگان
Jun-Seop Shin, Jae-Jeong Lee, Eun-Ju Lee, Yeon-Hee Kim, Kwon-Seok Chae, Chan-Wha Kim,