Molecular structure of large-scale extracted β-glucan from barley and oat: Identification of a significantly changed block structure in a high β-glucan barley mutant

► We elucidate the molecular structure of large-scale extracted barley and oat β-glucans. ► Mutant barley β-glucan has a unique oligomer block structure compared to normal barley. ► We model the β-glucan oligomer profiles from NMR spectroscopic data. ► Low solubility of barley β-glucans is an effect of a high number of DP3 oligomer blocks. ► Complex relations of wide-ranging β-glucan data is elucidated by multivariate data analysis.