Enantioseparation of 2-O-β-d-glucopyranosyl-2H-1,4-benzoxazin-3(4H)-one and its 7-chloro-derivative by capillary zone electrophoresis using native and substituted β-cyclodextrins as chiral additives

Efficient, rapid and inexpensive methods were established for the chiral separation of two glucopyranosyl compounds from plant extracts, by capillary zone electrophoresis (CZE). Baseline separation was achieved for both compounds. Several native cyclodextrins and their derivatives were tried as chiral selectors. CM-β-CD and HP-β-CD (with addition of acetonitrile in the buffer) gave rise to optimal chiral separation for the two compounds, respectively, each within a few minutes. The effects of several parameters on the chiral separation were studied.