کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10552212 | 967192 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mass spectrometric analysis of integral membrane proteins at the subpicomolar level: Application to rhodopsin
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
LTQOpsin - اپسیسProtein sequencing - توالی پروتئینPigment - رنگدانهRhodopsin - رودوپسینProtein identification - شناسایی پروتئینMass spectrometry - طیف سنجی جرمیProtein mapping - نقشه برداری پروتئینMembrane protein - پروتئین غشائیHPLC - کروماتوگرافی مایعی کاراG protein-coupled receptor - گیرندههای جفتشونده با پروتئین جی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Integral membrane proteins are among the most interesting molecules for biomedical research, as some of the most important cellular functions are inherently tied to biological membranes. One such example is the vast expanse of receptors on cell surfaces. However, due to difficulties in the biochemical purification and structure/function analysis of membrane proteins, caused by their hydrophobic or amphophilic nature, membrane proteins are still much less studied than soluble proteins. Our laboratory has successfully developed and applied a methodology for the mass spectrometric analysis of integral membrane proteins. Here, we present an improvement in the sensitivity of detection made possible by the advancement of mass spectrometric instrumentation and refinement of the chromatographic analysis. Subpicomolar samples of bovine rhodopsin purified from native membranes were successfully analyzed, obtaining complete sequence coverage and the detection and localization of posttranslational modifications. Therefore, it is demonstrated that the detection limits and sequence coverage for soluble and membrane proteins can be comparable. The methodology presented here allows mass spectrometric analysis of subpicomolar levels of photopigments or other integral membrane proteins either from their native membranes or as products of expression systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 825, Issue 2, 25 October 2005, Pages 169-175
Journal: Journal of Chromatography B - Volume 825, Issue 2, 25 October 2005, Pages 169-175
نویسندگان
Zsolt Ablonczy, Rosalie K. Crouch, Daniel R. Knapp,