کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10553414 | 967763 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Angiotensin-I converting enzyme (ACE) inhibitory tripeptides from rice protein hydrolysate: Purification and characterization
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کلمات کلیدی
RP-HPLCphenylthiohydantoinInhibition patternHHLpTHTFAACESBPAngiotensin-I Converting Enzyme - آنژیوتانسین I تبدیل آنزیمAntihypertensive effect - اثر ضد فشار خونTrifluoroacetic acid - اسید TrifluoroaceticHippuric acid - اسید هیپوریکbed volume - حجم تختSpontaneously Hypertensive Rat - خودآزمایی موش بالینیdegree of hydrolysis - درجه هیدرولیزShr - شریsystolic blood pressure - فشار خون سیستولیکPurification - پاکسازیACE-inhibitory peptides - پپتیدهای مهار کننده ACEReversed phase high performance liquid chromatography - کروماتوگرافی مایع با عملکرد بالا فاز برگشت پذیرhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, angiotensin-I converting enzyme (ACE) inhibitory tripeptides from rice protein hydrolysate were purified and their properties were further characterized. Val-Asn-Pro (VNP) and Val-Trp-Pro (VWP) were successfully purified from rice protein hydrolysate through multi-step isolation and purification comprising of ultrafiltration and DA201-C macroporous adsorption resin, followed by a two-step reverse phase high performance liquid chromatography (RP-HPLC). The sequences of tripeptides were separately assayed by using automated protein/peptide sequencer. In addition, the stability of the tripeptides against ACE and simulated gastrointestinal proteases, ACE-inhibitory kinetics, and their antihypertensive effects in vivo were evaluated. Our results showed that VNP (IC50 value of 6.4 μM) and VWP (IC50 value of 4.5 μM) were both competitive ACE inhibitors, and stability against ACE and gastrointestinal proteases of pepsin and chymotrypsin. Furthermore, single oral administration of the tripeptides VNP and VWP at 5 mg/kg body weight in spontaneously hypertensive rats (SHRs) significantly decreased the systolic blood pressure (SBP) of 29 and 38 mmHg (P < 0.05), respectively, and the antihypertensive effects of tripeptides could last for 8 h minimally. The identified unique characterization of VNP and VWP may enable the application of rice protein hydrolysate as functional foods or pharmaceuticals against hypertension.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 5, Issue 4, October 2013, Pages 1684-1692
Journal: Journal of Functional Foods - Volume 5, Issue 4, October 2013, Pages 1684-1692
نویسندگان
Jiwang Chen, Shanshan Liu, Ran Ye, Guangxia Cai, Bo Ji, Yongning Wu,