کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10553566 | 967830 | 2011 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-performance liquid chromatography assay of gnetol in rat serum and application to pre-clinical pharmacokinetic studies
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
R.S.D.peak area ratioS.E.M.StilbeneLOQDMSO - DMSOReversed-phase HPLC - HPLC فاز عقبUltraviolet - اشعه فرابنفشrelative standard deviation - انحراف استاندارد نسبیUV-detection - تشخیص UVPar - توسطVolume of distribution - حجم توزیعstandard error of the mean - خطای استاندارد میانگینDimethyl sulfoxide - دیمتیل سولفواکسیدPharmacokinetics - فارماکوکینتیکlimit of quantitation - محدودیت مقدارhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High-performance liquid chromatography assay of gnetol in rat serum and application to pre-clinical pharmacokinetic studies High-performance liquid chromatography assay of gnetol in rat serum and application to pre-clinical pharmacokinetic studies](/preview/png/10553566.png)
چکیده انگلیسی
A method of analysis of gnetol (2,3â²,5â²,6-tetrahydroxy-trans-stilbene) in biological fluids is necessary for the study of its kinetics and disposition in plants. A simple high-performance liquid chromatography (HPLC) method was developed for the determination of gnetol in rat serum using a reverse-phase, isocratic system. Separation was achieved using a Phenomenex® Luna C18 (2) column with ultraviolet detection at 305 nm. The standard curves were linear ranging from 0.5 to 100 μg/ml. The mean extraction efficiency was >90.5%. Precision of the assay was <14% (R.S.D.), and was within 14% at the limit of quantitation (0.5 μg/ml). Bias of the assay was lower than 15%, and was within 15% at the limit of quantitation. The assay was applied successfully to the study of serum disposition of gnetol in rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 54, Issue 4, 25 March 2011, Pages 878-881
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 54, Issue 4, 25 March 2011, Pages 878-881
نویسندگان
Connie M. Remsberg, Jody K. Takemoto, Rebecca M. Bertram, Neal M. Davies,