کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10553699 | 967909 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
RP-HPLC determination of recombinant human interferon omega in the Pichia pastoris fermentation broth
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: RP-HPLC determination of recombinant human interferon omega in the Pichia pastoris fermentation broth RP-HPLC determination of recombinant human interferon omega in the Pichia pastoris fermentation broth](/preview/png/10553699.png)
چکیده انگلیسی
A rapid and valid reversed-phase high performance liquid chromatography (RP-HPLC) method for determination of recombinant human interferon omega (rhIFNÏ) in the yeast Pichia pastoris fermentation broth was developed. The method is based on the hydrophobicity of rhIFNÏ followed by RP-HPLC separation with UV detection. The chromatography analysis was performed on EC 250/4 NUCLEOSIL 300-5 C18 (250 mm Ã 4 mm i.d., 300 Ã
, with a particle size of 5 μm) column. The compositions of the mobile phase A and B were 999:1 (v/v) water/TFA and 999:1 (v/v) acetonitrile/TFA at a flow rate of 1.0 ml minâ1. Detection was done by spectrophotometry at 280 nm and the column temperature was 30 ± 1 °C. Calibration curve was linear (r = 0.9986, n = 7) in the range of 0.074-0.555 mg mlâ1 for rhIFNÏ and the regression equation was y = 2.02 Ã 106 x â 1.27 Ã 105. Limit of detection for rhIFNÏ was 0.053 mg mlâ1. The values of R.S.D. (%) of intra-day and inter-day precision were <5.65 and <5.68 (n = 6), respectively. The R.S.D. (%) values and the average recovery rate of recovery experiment were <1.23 (n = 3) and 97.97%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 4, 15 July 2005, Pages 734-737
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 4, 15 July 2005, Pages 734-737
نویسندگان
Hong Liu, Hong-Chun Pan, Li Peng, Shao-Xi Cai,