Identification of in vivo protein phosphorylation sites in human pathogen Schistosoma japonicum by a phosphoproteomic approach

► in vivo protein phosphorylation sites were determined in Schistosoma japonicum. ► A phosphoproteomic approach was applied in two different stages of schistosomes. ► We identified 127 distinct phosphorylation sites in 92 proteins in S. japonicum. ► 30 phosphoproteins were shown to be co-detected in both the two stages. ► These co-detected phosphoproteins included several signal molecules and enzymes.