Fibrinogen alpha chain O-glycopeptides as possible markers of urinary tract infection

► We performed the first exploratory LC-MS study of human urine in the context of febrile, complicated urinary tract infection. ► Multivariate data analysis revealed O-glycopeptides as the main UTI specific features. ► The identification of those features as O-glycosilated fibrinogen alpha chain fragments required a multi-MS platform approach. ► We suggest that these O-glycosilated fibrinogen alpha chain fragments could be possible markers of febrile UTI.