Multiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cells

► Tubeimoside-1 could induce apoptosis of cancer cells. ► we examine changes of protein expression by fractionation proteomics. ► Tubeimoside-1 induced ROS generation and Ca2+ burden that further mediated intracellular stresses in mitochondria, ER and cytoskeleton organelles. ► Tubeimoside-1 induced G2/M cell cycle arrest in different cell lines.