کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10560639 | 969739 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid enzymatic chemiluminescent assay of glucose by means of a hybrid flow-injection/sequential-injection method
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rapid enzymatic chemiluminescent assay of glucose by means of a hybrid flow-injection/sequential-injection method Rapid enzymatic chemiluminescent assay of glucose by means of a hybrid flow-injection/sequential-injection method](/preview/png/10560639.png)
چکیده انگلیسی
This work reports a hybrid flow-injection analysis (FIA)/sequential-injection analysis (SIA) method for the rapid enzymatic assay of glucose with soluble glucose oxidase (GOD). The method relies on the sequential injection of segments of the sample and of a solution of enzyme by means of a multi-port selection valve in a flowing water stream. As the two zones are swept downstream, they overlap and merge so that the glucose in the sample is enzymatically oxidised. The generated hydrogen peroxide is merged with an alkaline luminol solution and the chemiluminescence (CL) intensity is monitored and related to the glucose concentration in the sample. The linear range of the method for glucose determination is 0.01-1 mmol Lâ1, the relative standard deviation is 3.9% at the 0.08 mmol Lâ1 level (n = 8), the limit of detection at the 2Ï level is 4 μmol Lâ1 glucose and the injection rate is 80 hâ1. The method was applied to the analysis of energy drinks and honey with relative errors in glucose determination in the range 100 ± 4.3%. The advantages of the proposed method are the wide linear range, the simple instrumentation used, the low consumption of sample and reagents, the elimination of catalysts and immobilised enzymes and the high sample throughput.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 67, Issue 3, 15 September 2005, Pages 603-609
Journal: Talanta - Volume 67, Issue 3, 15 September 2005, Pages 603-609
نویسندگان
Polyxeni Panoutsou, Anastasios Economou,